Classical anticytokinins do not interact with cytokinin receptors, but inhibit cyclin-dependent kinases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004793" target="_blank" >RIV/61989592:15310/07:00004793 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/07:00099660 RIV/61389030:_____/07:00099660

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Classical anticytokinins do not interact with cytokinin receptors, but inhibit cyclin-dependent kinases

Popis výsledku v původním jazyce

Cytokinins are a class of plant hormones that regulate the cell cycle and diverse developmental and physiological processes. Several compounds have been identified that antagonise the effects of cytokinins. Based on structural similarities and competitive inhibition, it has been assumed that these anticytokinins act through a common cellular target, namely the cytokinin receptor. Here, we examined directly, the possibility that various representative classical anticytokinins inhibit the Arabidopsis cytokinin receptors CRE1/AHK4 and AHK3. We show that pyrrolo[2,3-d]pyrimidine and pyrazolo[4,3-d]pyrimidine anticytokinins do not act as competitors of cytokinins at the receptor level. Flow cytometry and microscopic analyses revealed that anticytokinins inhibit the cell cycle and cause disorganisation of the microtubular cytoskeleton and apoptosis. This is consistent with the hypothesis that they inhibit regulatory CDK enzymes. Biochemical studies demonstrated inhibition by selected anticyt

Název v anglickém jazyce

Classical anticytokinins do not interact with cytokinin receptors, but inhibit cyclin-dependent kinases

Popis výsledku anglicky

Cytokinins are a class of plant hormones that regulate the cell cycle and diverse developmental and physiological processes. Several compounds have been identified that antagonise the effects of cytokinins. Based on structural similarities and competitive inhibition, it has been assumed that these anticytokinins act through a common cellular target, namely the cytokinin receptor. Here, we examined directly, the possibility that various representative classical anticytokinins inhibit the Arabidopsis cytokinin receptors CRE1/AHK4 and AHK3. We show that pyrrolo[2,3-d]pyrimidine and pyrazolo[4,3-d]pyrimidine anticytokinins do not act as competitors of cytokinins at the receptor level. Flow cytometry and microscopic analyses revealed that anticytokinins inhibit the cell cycle and cause disorganisation of the microtubular cytoskeleton and apoptosis. This is consistent with the hypothesis that they inhibit regulatory CDK enzymes. Biochemical studies demonstrated inhibition by selected anticyt

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Svazek periodika

282

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

14356-14363

Kód UT WoS článku

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EID výsledku v databázi Scopus

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