Imunoafinitní chromatografie kyseliny abscisové kombinovaná s kapalinovou chromotatografií-hmotnostní spektrometrií.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004919" target="_blank" >RIV/61989592:15310/07:00004919 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/07:00305972

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography?mass spectrometry.

Popis výsledku v původním jazyce

Polyclonal antibodies with high specificity for C1-immobilised (+)-cis,trans-abscisic acid (ABA) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of an immunoaffinity chromatography (IAC) gel. The detectionlimit of the ELISA was approximately 4.6x10-10 mol/L. Sensitive electrospray liquid chromatography?mass spectrometry (LC?ESI-MS) methods were also developed with detection limits below 0.1x10e-12 mol. The IAC allowed quick, single-step processing of samples prior to the analyses. The LC?ESI-MS and LC?ELISA techniques were used for comparative estimation of endogenous ABA levels in immunoaffinity purified extracts of normal and water-stressed Nicotiana tabacum L. leaves. The analytical approaches were validated using deuterium- and tritium-labelled internal standards, respectively. The IAC method was found to be highly effective, sensitive and convenient for isolating the target analyte from plant material.

Název v anglickém jazyce

Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography?mass spectrometry.

Popis výsledku anglicky

Polyclonal antibodies with high specificity for C1-immobilised (+)-cis,trans-abscisic acid (ABA) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of an immunoaffinity chromatography (IAC) gel. The detectionlimit of the ELISA was approximately 4.6x10-10 mol/L. Sensitive electrospray liquid chromatography?mass spectrometry (LC?ESI-MS) methods were also developed with detection limits below 0.1x10e-12 mol. The IAC allowed quick, single-step processing of samples prior to the analyses. The LC?ESI-MS and LC?ELISA techniques were used for comparative estimation of endogenous ABA levels in immunoaffinity purified extracts of normal and water-stressed Nicotiana tabacum L. leaves. The analytical approaches were validated using deuterium- and tritium-labelled internal standards, respectively. The IAC method was found to be highly effective, sensitive and convenient for isolating the target analyte from plant material.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Svazek periodika

847

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

162-173

Kód UT WoS článku

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EID výsledku v databázi Scopus

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