Kvantitativní nano zkoušky založené na kapilární elektroforéze pro monitorování ecto-5´-nukleotidasové aktivity a inhibice v přípravě rekombinovaných enzymů a melanomů buněčných membrán

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005793" target="_blank" >RIV/61989592:15310/08:00005793 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Capillary electrophoresis-based nanoscale assays for monitoring ecto-5 '-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes

Popis výsledku v původním jazyce

Powerful capillary electrophoresis (CE) methods were developed for monitoring the reaction of ecto-5'-nucleotidase (ecto-5'-NT, CD73), a (path o)biochemically important enzyme that hydrolyzes nucleo side- 5'-monophosphates to the corresponding nucleosides. The enzymatic reaction was performed either before injection into the capillary (method A) or directly within the capillary (method 13). In method A, separation of substrates and products was achieved within 8 min using an eCAP fused-silica capillary(20 cm effective length, 75 mu M i.d., UV detection at 260 nm), 40 mM sodium borate buffer (pH 9.1), normal polarity, and a constant voltage of 15 kV. In method B, the sandwich technique was applied; substrate dissolved in reaction buffer (10 mM Hepes [pH 7.4], 2 mM MgCl2, and 1 mM CaCl2) was hydrodynamically injected into a fused-silica capillary (30 cm, 75 M i.d.), followed by enzyme (recombinant rat ecto-5'-NT) and subsequent injection of substrate solution. The reaction was initi

Název v anglickém jazyce

Capillary electrophoresis-based nanoscale assays for monitoring ecto-5 '-nucleotidase activity and inhibition in preparations of recombinant enzyme and melanoma cell membranes

Popis výsledku anglicky

Powerful capillary electrophoresis (CE) methods were developed for monitoring the reaction of ecto-5'-nucleotidase (ecto-5'-NT, CD73), a (path o)biochemically important enzyme that hydrolyzes nucleo side- 5'-monophosphates to the corresponding nucleosides. The enzymatic reaction was performed either before injection into the capillary (method A) or directly within the capillary (method 13). In method A, separation of substrates and products was achieved within 8 min using an eCAP fused-silica capillary(20 cm effective length, 75 mu M i.d., UV detection at 260 nm), 40 mM sodium borate buffer (pH 9.1), normal polarity, and a constant voltage of 15 kV. In method B, the sandwich technique was applied; substrate dissolved in reaction buffer (10 mM Hepes [pH 7.4], 2 mM MgCl2, and 1 mM CaCl2) was hydrodynamically injected into a fused-silica capillary (30 cm, 75 M i.d.), followed by enzyme (recombinant rat ecto-5'-NT) and subsequent injection of substrate solution. The reaction was initi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Biochemistry

ISSN

0003-2697

e-ISSN

—

Svazek periodika

373

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—