Factors affecting DNA-DNA interstrand cross-links in the antiparallel 3'-3' sense: A comparison with the 5'-5' directional isomer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010122" target="_blank" >RIV/61989592:15310/09:00010122 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/09:00330736

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Factors affecting DNA-DNA interstrand cross-links in the antiparallel 3'-3' sense: A comparison with the 5'-5' directional isomer

Popis výsledku v původním jazyce

Reported herein is a study of the unusual 3'-3' 1,4-GG interstrand cross-link (IXL) formation in duplex DNA by a series of polynuclear platinum anticancer complexes. To examine the effect of possible preassociation through charge and hydrogen-bonding effects the closely related compounds [{trans-PtCl(NH3)2}2(-trans-Pt(NH3)2-{NH2 (CH2)6NH2}2)]4+ (BBR3464, 1), [{trans-PtCl (NH3)2}2 (-NH2 (CH2)6-NH2)]2+ (BBR3005, 2), [{trans-PtCl(NH3)2}2(-H2N (CH2)3NH2 (CH2)4)]3+ (BBR3571, 3) and [{trans-PtCl (NH3)2}2-{-H2N (CH2)3N (COCF3) (CH2)4}]2+ (BBR3571-COCF3, 4) were studied. Two different molecular biology approaches were used to investigate the effect of DNA template upon IXL formation in synthetic 20-base-pair duplexes. In the "hybridisation directed" method themonofunctionally adducted top strands were hybridised with their complementary 5'-end labelled strands; after 24 h the efficiency of interstrand cross-linking in the 5'-5' direction was slightly higher than in the 3

Název v anglickém jazyce

Factors affecting DNA-DNA interstrand cross-links in the antiparallel 3'-3' sense: A comparison with the 5'-5' directional isomer

Popis výsledku anglicky

Reported herein is a study of the unusual 3'-3' 1,4-GG interstrand cross-link (IXL) formation in duplex DNA by a series of polynuclear platinum anticancer complexes. To examine the effect of possible preassociation through charge and hydrogen-bonding effects the closely related compounds [{trans-PtCl(NH3)2}2(-trans-Pt(NH3)2-{NH2 (CH2)6NH2}2)]4+ (BBR3464, 1), [{trans-PtCl (NH3)2}2 (-NH2 (CH2)6-NH2)]2+ (BBR3005, 2), [{trans-PtCl(NH3)2}2(-H2N (CH2)3NH2 (CH2)4)]3+ (BBR3571, 3) and [{trans-PtCl (NH3)2}2-{-H2N (CH2)3N (COCF3) (CH2)4}]2+ (BBR3571-COCF3, 4) were studied. Two different molecular biology approaches were used to investigate the effect of DNA template upon IXL formation in synthetic 20-base-pair duplexes. In the "hybridisation directed" method themonofunctionally adducted top strands were hybridised with their complementary 5'-end labelled strands; after 24 h the efficiency of interstrand cross-linking in the 5'-5' direction was slightly higher than in the 3

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemistry: A European Journal

ISSN

0947-6539

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

37

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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