A comparison of DNA binding profiles of dinuclear platinum compounds with polyamine linkers and the trinuclear platinum phase II clinical agent BBR3464.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F02%3A17023025" target="_blank" >RIV/68081707:_____/02:17023025 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/02:00007654

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A comparison of DNA binding profiles of dinuclear platinum compounds with polyamine linkers and the trinuclear platinum phase II clinical agent BBR3464.

Popis výsledku v původním jazyce

The DNA binding profiles of three bis Pt(II) polyamine-linked compounds, [{trans-PtCl(NH3)(2)}(2) {mu-spermine-N-1,N-12}](4+), [{trans-PtCl(NH3)(2)}(2){mu-spermidine-N-1,N-8}](3+) and [{trans-PtCl(NH3)(2)}(2){mu-BOC-spermidine}](2+), were compared with that of a novel trinuclear phase II clinical agent, [{trans-PtCl(NH3)(2)}(2){mu-trans-Pt(NH3)(2)(H2N(CH2)(6)NH2)(2)}](4+). All of the compounds bind preferentially in a bifunctional manner, according to unwinding of supercoiled DNA circles. The kinetics of binding of these compounds corresponds to their relative charge (2 + to 4 +). The preference for the formation of interstrand crosslinks, however, does not follow a charge-based pattern. By studying the results of DNA polymerase extension products on aDNA template modified by the compounds, and by incorporating the complementary method of RNA transcription mapping, it was possible to determine the nucleotide bases that are preferred sites of binding.

Název v anglickém jazyce

A comparison of DNA binding profiles of dinuclear platinum compounds with polyamine linkers and the trinuclear platinum phase II clinical agent BBR3464.

Popis výsledku anglicky

The DNA binding profiles of three bis Pt(II) polyamine-linked compounds, [{trans-PtCl(NH3)(2)}(2) {mu-spermine-N-1,N-12}](4+), [{trans-PtCl(NH3)(2)}(2){mu-spermidine-N-1,N-8}](3+) and [{trans-PtCl(NH3)(2)}(2){mu-BOC-spermidine}](2+), were compared with that of a novel trinuclear phase II clinical agent, [{trans-PtCl(NH3)(2)}(2){mu-trans-Pt(NH3)(2)(H2N(CH2)(6)NH2)(2)}](4+). All of the compounds bind preferentially in a bifunctional manner, according to unwinding of supercoiled DNA circles. The kinetics of binding of these compounds corresponds to their relative charge (2 + to 4 +). The preference for the formation of interstrand crosslinks, however, does not follow a charge-based pattern. By studying the results of DNA polymerase extension products on aDNA template modified by the compounds, and by incorporating the complementary method of RNA transcription mapping, it was possible to determine the nucleotide bases that are preferred sites of binding.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Inorganic Chemistry

ISSN

0949-8257

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

4/5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

397-404

Kód UT WoS článku

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EID výsledku v databázi Scopus

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