Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010322" target="_blank" >RIV/61989592:15310/09:00010322 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize

Popis výsledku v původním jazyce

The aim was to characterize the maize non-secreted CKX gene and elucidate the ZmCKX10 subcellular localization. For determination of subcellular localization, the CKX genes were fused with green fluorescent protein (GFP) and overexpressed in tomato hairyroots. Using confocal microscopy, ZmCKX1-GFP signal was confirmed to be present in the apoplast, whereas ZmCKX10-GFP was detected in the cytosol. While roots overexpressing ZmCKX1-GFP formed significantly more mass in comparison to the control, non-secreted CKX overexpression resulted in a small reduction in root mass accumulation. Biochemical characterization of ZmCKX10 was performed using recombinant protein produced in Pichia pastoris. In contrast to the preference for 2,6-dichlorophenolindophenol (DCPIP) as an electron acceptor and transzeatin, N6-(?2-isopentenyl)adenine (iP) and N6-(? 2-isopentenyl)adenosin (iPR) as substrates for ZmCKX1, the non-secreted ZmCKX10 had a range of suitable electron acceptors and different substrate p

Název v anglickém jazyce

Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize

Popis výsledku anglicky

The aim was to characterize the maize non-secreted CKX gene and elucidate the ZmCKX10 subcellular localization. For determination of subcellular localization, the CKX genes were fused with green fluorescent protein (GFP) and overexpressed in tomato hairyroots. Using confocal microscopy, ZmCKX1-GFP signal was confirmed to be present in the apoplast, whereas ZmCKX10-GFP was detected in the cytosol. While roots overexpressing ZmCKX1-GFP formed significantly more mass in comparison to the control, non-secreted CKX overexpression resulted in a small reduction in root mass accumulation. Biochemical characterization of ZmCKX10 was performed using recombinant protein produced in Pichia pastoris. In contrast to the preference for 2,6-dichlorophenolindophenol (DCPIP) as an electron acceptor and transzeatin, N6-(?2-isopentenyl)adenine (iP) and N6-(? 2-isopentenyl)adenosin (iPR) as substrates for ZmCKX1, the non-secreted ZmCKX10 had a range of suitable electron acceptors and different substrate p

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ME%20861" target="_blank" >ME 861: Biochemické a strukturní porovnání dvou rozdílně cílených cytokinindehydrogenáz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Experimenal Botany

ISSN

0022-0957

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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