MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33116273" target="_blank" >RIV/61989592:15310/11:33116273 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10337-011-2019-1" target="_blank" >http://dx.doi.org/10.1007/s10337-011-2019-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-011-2019-1" target="_blank" >10.1007/s10337-011-2019-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix

Popis výsledku v původním jazyce

The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaClwas applied. The limit of detection after the preconcentration step for nilutamide was 26 mu g L(-1).

Název v anglickém jazyce

MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix

Popis výsledku anglicky

The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaClwas applied. The limit of detection after the preconcentration step for nilutamide was 26 mu g L(-1).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regionální centrum pokročilých technologií a materiálů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chromatographia

ISSN

0009-5893

e-ISSN

—

Svazek periodika

74

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

151-155

Kód UT WoS článku

000291745400019

EID výsledku v databázi Scopus

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