Electrochemical Determination of Transmembrane Protein Na+/K+ ATPase and Its Cytoplasmic Loop C45

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/61989592:15110/12:33139119

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Determination of Transmembrane Protein Na+/K+ ATPase and Its Cytoplasmic Loop C45

Popis výsledku v původním jazyce

Electrochemistry of membrane proteins is complicated by the fact that the studied substances are poorly soluble or insoluble in aqueous environment. The solubilization of proteins using surfactants (detergents) affects the electrochemical analysis or even renders it impossible. In the present study, the electrochemistry of the transmembrane protein Na+/K+-ATPase (NKA) and its water-soluble isolated cytoplasmic loop C45 is described. The proteins were studied using adsorptive transfer cyclic voltammetryand square-wave voltammetry on basal-plane pyrolytic graphite electrode (PGE) as well as constant-current chronopotentiometric stripping analysis on hanging mercury drop electrode (HMDE). The nonionic surfactant octaethylene glycol monododecyl ether (C12E8) was used for NKA solubilization. Under these conditions the oxidation currents of Tyr and Trp (peak Y: +0.55 V and peak W: +0.7 V, vs. Ag/ AgCl/3 M KCl) and catalytic reduction currents (peak H: 1.8 V) of NKA and C45 loop can be obser

Název v anglickém jazyce

Electrochemical Determination of Transmembrane Protein Na+/K+ ATPase and Its Cytoplasmic Loop C45

Popis výsledku anglicky

Electrochemistry of membrane proteins is complicated by the fact that the studied substances are poorly soluble or insoluble in aqueous environment. The solubilization of proteins using surfactants (detergents) affects the electrochemical analysis or even renders it impossible. In the present study, the electrochemistry of the transmembrane protein Na+/K+-ATPase (NKA) and its water-soluble isolated cytoplasmic loop C45 is described. The proteins were studied using adsorptive transfer cyclic voltammetryand square-wave voltammetry on basal-plane pyrolytic graphite electrode (PGE) as well as constant-current chronopotentiometric stripping analysis on hanging mercury drop electrode (HMDE). The nonionic surfactant octaethylene glycol monododecyl ether (C12E8) was used for NKA solubilization. Under these conditions the oxidation currents of Tyr and Trp (peak Y: +0.55 V and peak W: +0.7 V, vs. Ag/ AgCl/3 M KCl) and catalytic reduction currents (peak H: 1.8 V) of NKA and C45 loop can be obser

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

"1758 ? 1765"

Kód UT WoS článku

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EID výsledku v databázi Scopus

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