Construction and characterization of a reporter gene cell line for assessment of human glucocorticoid receptor activation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142474" target="_blank" >RIV/61989592:15310/12:33142474 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/12:10124917

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0928098712003855" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0928098712003855</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejps.2012.10.003" target="_blank" >10.1016/j.ejps.2012.10.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Construction and characterization of a reporter gene cell line for assessment of human glucocorticoid receptor activation

Popis výsledku v původním jazyce

Glucocorticoids are widely used drugs in human pharmacotherapy. There is an increasing demand for tools allowing detection of the ligands for glucocorticoid receptor (GR), with regard to pre-clinical drug testing and environmental applications. We constructed human luciferase reporter gene cell line AZ-GR derived from HeLa human cervix carcinoma cells, which were stably transfected with reporter plasmid containing three copies of glucorticoid response element (GRE) upstream of luciferase reporter gene.We isolated five dexamethasone-responsive clones, and we further characterized two most responsive ones (AZ-GR). Dose-response analyses were performed with 22 different natural and synthetic steroids and the values of EC50 were calculated. AZ-GR cells displayed high specificity and sensitivity to glucocorticoids, very low responsiveness to mineralocorticoids, but no responsiveness to estrogens, gestagens or androgens. Time-course analyses revealed that AZ-GR cells allow detection of GR a

Název v anglickém jazyce

Construction and characterization of a reporter gene cell line for assessment of human glucocorticoid receptor activation

Popis výsledku anglicky

Glucocorticoids are widely used drugs in human pharmacotherapy. There is an increasing demand for tools allowing detection of the ligands for glucocorticoid receptor (GR), with regard to pre-clinical drug testing and environmental applications. We constructed human luciferase reporter gene cell line AZ-GR derived from HeLa human cervix carcinoma cells, which were stably transfected with reporter plasmid containing three copies of glucorticoid response element (GRE) upstream of luciferase reporter gene.We isolated five dexamethasone-responsive clones, and we further characterized two most responsive ones (AZ-GR). Dose-response analyses were performed with 22 different natural and synthetic steroids and the values of EC50 were calculated. AZ-GR cells displayed high specificity and sensitivity to glucocorticoids, very low responsiveness to mineralocorticoids, but no responsiveness to estrogens, gestagens or androgens. Time-course analyses revealed that AZ-GR cells allow detection of GR a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Pharmaceutical Sciences

ISSN

0928-0987

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

842-847

Kód UT WoS článku

000313385100006

EID výsledku v databázi Scopus

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