Methanogens and methanotrophs distribution in the hyporheic sediments of a small lowland stream

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33143108" target="_blank" >RIV/61989592:15310/12:33143108 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1127/1863-9135/2012/0283" target="_blank" >http://dx.doi.org/10.1127/1863-9135/2012/0283</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1127/1863-9135/2012/0283" target="_blank" >10.1127/1863-9135/2012/0283</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Methanogens and methanotrophs distribution in the hyporheic sediments of a small lowland stream

Popis výsledku v původním jazyce

Distribution of microbial activity, methanogenic archaea and type I and II methanotrophs were studied in a small lowland stream Sitka in Czech Republic. The methanogens an methanotrophic bacteria were detected using FISH with 16 S rRNA-targeted oligonucleotide probes. The highest microbial density was obtained in the upper sediment layer 0 - 25 cm; this zone corresponded also to that of the highest metabolic activity, as indicated by the methanogenic potential, methanotrophic activity, INT and FDA profiles. Both methanogenic archaea and aerobic methanotrophs were found at all localities along the longitudinal stream profile. The proportion of these groups to the DAPI-stained cells was quite consistent and varied only slightly but a higher proportion tothe DAPI-stained cells in the deeper sediment layer 25 - 50 cm was observed. On average 23.4 % of DAPI-stained cells were detected by FISH with a probe for methanogens while type I methanotrophs reached ~ 21.4 % and type II methanotrophs

Název v anglickém jazyce

Methanogens and methanotrophs distribution in the hyporheic sediments of a small lowland stream

Popis výsledku anglicky

Distribution of microbial activity, methanogenic archaea and type I and II methanotrophs were studied in a small lowland stream Sitka in Czech Republic. The methanogens an methanotrophic bacteria were detected using FISH with 16 S rRNA-targeted oligonucleotide probes. The highest microbial density was obtained in the upper sediment layer 0 - 25 cm; this zone corresponded also to that of the highest metabolic activity, as indicated by the methanogenic potential, methanotrophic activity, INT and FDA profiles. Both methanogenic archaea and aerobic methanotrophs were found at all localities along the longitudinal stream profile. The proportion of these groups to the DAPI-stained cells was quite consistent and varied only slightly but a higher proportion tothe DAPI-stained cells in the deeper sediment layer 25 - 50 cm was observed. On average 23.4 % of DAPI-stained cells were detected by FISH with a probe for methanogens while type I methanotrophs reached ~ 21.4 % and type II methanotrophs

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DA - Hydrologie a limnologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA526%2F09%2F1639" target="_blank" >GA526/09/1639: Biogeochemie metanu a detekce metanogenních a metanotrofních bakterií v říčních sedimentech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fundamental and Applied Limnology

ISSN

1863-9135

e-ISSN

—

Svazek periodika

2012

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

16

Strana od-do

87-102

Kód UT WoS článku

000308743600001

EID výsledku v databázi Scopus

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