Electrochemical determination of hydrogen peroxide production by isolated mitochondria: A novel nanocomposite carbon-maghemite nanoparticle electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33145799" target="_blank" >RIV/61989592:15310/13:33145799 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0925400512009574" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0925400512009574</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2012.09.044" target="_blank" >10.1016/j.snb.2012.09.044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical determination of hydrogen peroxide production by isolated mitochondria: A novel nanocomposite carbon-maghemite nanoparticle electrode

Popis výsledku v původním jazyce

A simple carbon paste electrode modified with novel maghemite (gama-Fe2O3) nanoparticles, characterized by a mean diameter of about 10 nm and specific surface properties (called surface active maghemite nanoparticles, SAMNs) has been developed. The electrode is able to catalyze the electro-reduction of hydrogen peroxide at low applied potentials (?0.1 V vs. SCE). In order to improve the electrocatalytic properties of the modified electrode and to protect electrode surface from fouling due to the presence of biological materials, a surfactant, namely cetyltrimethylammonium bromide, was introduced, as monomolecular layer, on electrode surface. At ?0.1 V, the sensitivity of the modified nanocomposite electrode was 58 nA mýM?1 cm?2, with a detection limitof 2.78 mýM, in the 0.01-1.5 mM H2O2 concentration range, revealing to be one of the most sensitive electrodes described in literature. The resulting electrode was used to determine native monoamine oxidase activity on intact mitochondria

Název v anglickém jazyce

Electrochemical determination of hydrogen peroxide production by isolated mitochondria: A novel nanocomposite carbon-maghemite nanoparticle electrode

Popis výsledku anglicky

A simple carbon paste electrode modified with novel maghemite (gama-Fe2O3) nanoparticles, characterized by a mean diameter of about 10 nm and specific surface properties (called surface active maghemite nanoparticles, SAMNs) has been developed. The electrode is able to catalyze the electro-reduction of hydrogen peroxide at low applied potentials (?0.1 V vs. SCE). In order to improve the electrocatalytic properties of the modified electrode and to protect electrode surface from fouling due to the presence of biological materials, a surfactant, namely cetyltrimethylammonium bromide, was introduced, as monomolecular layer, on electrode surface. At ?0.1 V, the sensitivity of the modified nanocomposite electrode was 58 nA mýM?1 cm?2, with a detection limitof 2.78 mýM, in the 0.01-1.5 mM H2O2 concentration range, revealing to be one of the most sensitive electrodes described in literature. The resulting electrode was used to determine native monoamine oxidase activity on intact mitochondria

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers

ISSN

0925-4005

e-ISSN

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Svazek periodika

176

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

315-322

Kód UT WoS článku

000319867500044

EID výsledku v databázi Scopus

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