Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33152646" target="_blank" >RIV/61989592:15310/14:33152646 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60077344:_____/14:00439433 RIV/00159816:_____/14:00061216 RIV/60076658:12520/14:43886922

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0968432814001565" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0968432814001565</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.micron.2014.08.001" target="_blank" >10.1016/j.micron.2014.08.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

Popis výsledku v původním jazyce

When developing new nanoparticles for bio-applications, it is important to fully characterize the nanoparticle's behavior in biological systems. The most common techniques employed for mapping nanoparticles inside cells include transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). These techniques entail passing an electron beam through a thin specimen. STEM or TEM imaging is often used for the detection of nanoparticles inside cellular organelles. However, lengthysample preparation is required (i.e., fixation, dehydration, drying, resin embedding, and cutting). In the present work, a new matrix (FTO glass) for biological samples was used and characterized by field emission scanning electron microscopy (FE-SEM) to generate images comparable to those obtained by TEM. Using FE-SEM, nanoparticle images were acquired inside endo/lysosomes without disruption of the cellular shape. Furthermore, the initial steps of nanoparticle incorporation into the c

Název v anglickém jazyce

Field emission scanning electron microscopy (FE-SEM) as an approach for nanoparticle detection inside cells

Popis výsledku anglicky

When developing new nanoparticles for bio-applications, it is important to fully characterize the nanoparticle's behavior in biological systems. The most common techniques employed for mapping nanoparticles inside cells include transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM). These techniques entail passing an electron beam through a thin specimen. STEM or TEM imaging is often used for the detection of nanoparticles inside cellular organelles. However, lengthysample preparation is required (i.e., fixation, dehydration, drying, resin embedding, and cutting). In the present work, a new matrix (FTO glass) for biological samples was used and characterized by field emission scanning electron microscopy (FE-SEM) to generate images comparable to those obtained by TEM. Using FE-SEM, nanoparticle images were acquired inside endo/lysosomes without disruption of the cellular shape. Furthermore, the initial steps of nanoparticle incorporation into the c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Micron

ISSN

0968-4328

e-ISSN

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Svazek periodika

67

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

149-154

Kód UT WoS článku

000343641300019

EID výsledku v databázi Scopus

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