De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156137" target="_blank" >RIV/61989592:15310/15:33156137 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12838/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12838/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/1462-2920.12838" target="_blank" >10.1111/1462-2920.12838</a>

Jazyk výsledku

angličtina

Název v původním jazyce

De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea

Popis výsledku v původním jazyce

Disease symptoms of some phytopathogenic fungi are associated with changes in cytokinin (CK) levels. Here, we show that the CK pro?le of ergot-infected rye plants is also altered, although no pronounced changes occur in the expression of the host plant'sCK biosynthesis genes. Instead, we demonstrate a clearly different mechanism: we report on the ?rst fungal de novo CK biosynthesis genes, prove their functions and constitute a biosynthetic pathway. The ergot fungus Claviceps purpurea produces substantial quantities of CKs in culture and, like plants, expresses enzymes containing the isopentenyltransferase and lonely guy domains necessary for de novo isopentenyladenine production. Uniquely, two of these domains are combined in one bifunctional enzyme,CpIPT-LOG, depicting a novel and potent mechanism for CK production. The fungus also forms trans-zeatin, a reaction catalysed by a CK-speci?c cytochrome P450 monooxygenase, which is encoded by cpp450 forming a small cluster with cpipt-log

Název v anglickém jazyce

De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea

Popis výsledku anglicky

Disease symptoms of some phytopathogenic fungi are associated with changes in cytokinin (CK) levels. Here, we show that the CK pro?le of ergot-infected rye plants is also altered, although no pronounced changes occur in the expression of the host plant'sCK biosynthesis genes. Instead, we demonstrate a clearly different mechanism: we report on the ?rst fungal de novo CK biosynthesis genes, prove their functions and constitute a biosynthetic pathway. The ergot fungus Claviceps purpurea produces substantial quantities of CKs in culture and, like plants, expresses enzymes containing the isopentenyltransferase and lonely guy domains necessary for de novo isopentenyladenine production. Uniquely, two of these domains are combined in one bifunctional enzyme,CpIPT-LOG, depicting a novel and potent mechanism for CK production. The fungus also forms trans-zeatin, a reaction catalysed by a CK-speci?c cytochrome P450 monooxygenase, which is encoded by cpp450 forming a small cluster with cpipt-log

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology

ISSN

1462-2920

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

2935-2951

Kód UT WoS článku

000360048800028

EID výsledku v databázi Scopus

—