Genome-wide analysis of the barley MAPK gene family and its expression patterns in relation to Puccinia hordei infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156610" target="_blank" >RIV/61989592:15310/15:33156610 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s11738-015-2010-9" target="_blank" >http://link.springer.com/article/10.1007/s11738-015-2010-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11738-015-2010-9" target="_blank" >10.1007/s11738-015-2010-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genome-wide analysis of the barley MAPK gene family and its expression patterns in relation to Puccinia hordei infection

Popis výsledku v původním jazyce

Mitogen-activated protein kinases (MAPKs) have been shown to act as key regulators of stress responses in model plant and crop species. So far, however, the MAPK family has not been systematically studied in barley. Herein, we identified 16 HvMAPKs (Hv-Hordeum vulgare) based on computational analysis of barley transcriptomics and genomics databases. HvMAPKs contain all canonical MAPK domains, except for HvMPK2, which lacks a MAPK domain signature. In addition, five HvMAPKs harbor TEY and ten HvMAPKs harbor TDY dual phosphorylation motif in the activation loop. Interestingly, HvMPK2 contains a MEY instead of TEY phosphorylation motif. We classified HvMAPKs into four major plant MAPK clades based on phylogeny reconstruction and anchored all HvMAPK genesto five out of seven barley chromosomes. Furthermore, we inoculated seedlings of susceptible barley line L94 and its isolines L94-Rph3 and L94-Rph7 with rust fungus Puccinia hordei and analyzed the expression of 16 HvMAPK genes using qRT-

Název v anglickém jazyce

Genome-wide analysis of the barley MAPK gene family and its expression patterns in relation to Puccinia hordei infection

Popis výsledku anglicky

Mitogen-activated protein kinases (MAPKs) have been shown to act as key regulators of stress responses in model plant and crop species. So far, however, the MAPK family has not been systematically studied in barley. Herein, we identified 16 HvMAPKs (Hv-Hordeum vulgare) based on computational analysis of barley transcriptomics and genomics databases. HvMAPKs contain all canonical MAPK domains, except for HvMPK2, which lacks a MAPK domain signature. In addition, five HvMAPKs harbor TEY and ten HvMAPKs harbor TDY dual phosphorylation motif in the activation loop. Interestingly, HvMPK2 contains a MEY instead of TEY phosphorylation motif. We classified HvMAPKs into four major plant MAPK clades based on phylogeny reconstruction and anchored all HvMAPK genesto five out of seven barley chromosomes. Furthermore, we inoculated seedlings of susceptible barley line L94 and its isolines L94-Rph3 and L94-Rph7 with rust fungus Puccinia hordei and analyzed the expression of 16 HvMAPK genes using qRT-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GPP501%2F12%2FP455" target="_blank" >GPP501/12/P455: Úloha mitogen-aktivovaných protein kináz (MPKs) v odpovědích ječmene na napadení Puccinia hordei</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

3.11.2015

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

16

Strana od-do

"254-1"-"254-16"

Kód UT WoS článku

000365020200035

EID výsledku v databázi Scopus

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