MicroRNA Regulation of Abiotic Stress Response in 7B-1 Male-Sterile Tomato Mutant
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33157528" target="_blank" >RIV/61989592:15310/15:33157528 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61389030:_____/15:00452703
Výsledek na webu
<a href="https://dl.sciencesocieties.org/publications/tpg/articles/8/3/plantgenome2015.02.0008" target="_blank" >https://dl.sciencesocieties.org/publications/tpg/articles/8/3/plantgenome2015.02.0008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3835/plantgenome2015.02.0008" target="_blank" >10.3835/plantgenome2015.02.0008</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
MicroRNA Regulation of Abiotic Stress Response in 7B-1 Male-Sterile Tomato Mutant
Popis výsledku v původním jazyce
The 7B-1 tomato (Solanum lycopersicum L. 'Rutgers') is a male-sterile mutant with enhanced tolerance to abiotic stress in a bluelight (BL) specific manner compared with its wild-type (WT). This makes the 7B-1 a potential candidate for hybrid seed breeding and stress engineering. To identify small RNAs (sRNAs) linked to stress tolerance of 7B-1, two sRNA libraries from BL-grown 7B-1 and WT seedlings treated simultaneously with abscisic acid (ABA) and mannitol were sequenced, and sRNA profiles were compared. Twenty nine families of known microRNAs (miRNAs) and 27 putative novel miRNAs were identified from the two libraries. MiR5300, miR5301, miR2916, and a novel miRNA denoted miR# C were upregulated, while miR159, miR166, miR472, miR482, and two novel miRNAs, miR# A and miR# D, were downregulated in stress-treated 7B-1 seedlings. MiRNA targets with potential roles in stress regulation were validated by rapid amplification of 5' complementary DNA ends (5'-RACE) analysis. Expression of miR
Název v anglickém jazyce
MicroRNA Regulation of Abiotic Stress Response in 7B-1 Male-Sterile Tomato Mutant
Popis výsledku anglicky
The 7B-1 tomato (Solanum lycopersicum L. 'Rutgers') is a male-sterile mutant with enhanced tolerance to abiotic stress in a bluelight (BL) specific manner compared with its wild-type (WT). This makes the 7B-1 a potential candidate for hybrid seed breeding and stress engineering. To identify small RNAs (sRNAs) linked to stress tolerance of 7B-1, two sRNA libraries from BL-grown 7B-1 and WT seedlings treated simultaneously with abscisic acid (ABA) and mannitol were sequenced, and sRNA profiles were compared. Twenty nine families of known microRNAs (miRNAs) and 27 putative novel miRNAs were identified from the two libraries. MiR5300, miR5301, miR2916, and a novel miRNA denoted miR# C were upregulated, while miR159, miR166, miR472, miR482, and two novel miRNAs, miR# A and miR# D, were downregulated in stress-treated 7B-1 seedlings. MiRNA targets with potential roles in stress regulation were validated by rapid amplification of 5' complementary DNA ends (5'-RACE) analysis. Expression of miR
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Genome
ISSN
1940-3372
e-ISSN
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Svazek periodika
8
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
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Kód UT WoS článku
000367390800005
EID výsledku v databázi Scopus
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