Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F17%3A73584066" target="_blank" >RIV/61989592:15310/17:73584066 - isvavai.cz</a>

Výsledek na webu

<a href="https://peerj.com/articles/3050/" target="_blank" >https://peerj.com/articles/3050/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7717/peerj.3050" target="_blank" >10.7717/peerj.3050</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study

Popis výsledku v původním jazyce

The growth and development of plants is deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O2), hydrogen peroxide (H2O2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O2 formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrine-adrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT); however, kinetic measurements have not been performed. In the current study, we provide evidence of O2 generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding. Real-time monitoring of O2 generation was performed using catalytic amperometry. Changes in oxidation current for O2 was monitored using polymeric iron-porphyrin-based modified carbon electrodes as working electrode with Ag/AgCl as the reference electrode. The results obtained show continuous generation of O2 for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O2 to H2O2, significantly suppressed the oxidation current. Catalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. We claim it to be a useful tool and a direct method for real-time monitoring and precise detection of O2 in biological samples, with the potential for wide application in plant research for specific and sensitive detection of O2.

Název v anglickém jazyce

Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study

Popis výsledku anglicky

The growth and development of plants is deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O2), hydrogen peroxide (H2O2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O2 formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrine-adrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT); however, kinetic measurements have not been performed. In the current study, we provide evidence of O2 generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding. Real-time monitoring of O2 generation was performed using catalytic amperometry. Changes in oxidation current for O2 was monitored using polymeric iron-porphyrin-based modified carbon electrodes as working electrode with Ag/AgCl as the reference electrode. The results obtained show continuous generation of O2 for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O2 to H2O2, significantly suppressed the oxidation current. Catalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. We claim it to be a useful tool and a direct method for real-time monitoring and precise detection of O2 in biological samples, with the potential for wide application in plant research for specific and sensitive detection of O2.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PeerJ

ISSN

2167-8359

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

JUL

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

"e3050-1"-"e3050-18"

Kód UT WoS článku

000406369500005

EID výsledku v databázi Scopus

—