Chapter 2 - Basis of mass spectrometry: Technical variants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73590208" target="_blank" >RIV/61989592:15310/18:73590208 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/B9780128144510000022" target="_blank" >https://www.sciencedirect.com/science/article/pii/B9780128144510000022</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-12-814451-0.00002-2" target="_blank" >10.1016/B978-0-12-814451-0.00002-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chapter 2 - Basis of mass spectrometry: Technical variants

Popis výsledku v původním jazyce

Chapter 2, Basis of Mass Spectrometry: Technical Variants, provides a definition of mass spectrometry (MS) and describes the history of this analytical technique from the very beginning. Then it summarizes basic components of a mass spectrometer, from which namely mass analyzers are further described in detail together with their combinations in hybrid mass spectrometers. Such instruments are designed to perform tandem MS, which has been used in chemistry and biology to identify and quantify compounds. All possible modes of tandem MS are mentioned as well as common ion activation techniques for the fragmentation of gas-phase ions, which is essential and occurs between different stages of mass analysis. Next, separation techniques hyphenated with MS are discussed, namely gas and liquid chromatography (GC and LC, respectively) and capillary electrophoresis (CE). Hyphenated systems are nowadays almost indispensable for investigations of complex biological samples containing proteins, lipids, or metabolites. Mass spectrometry imaging (MSI) is presented as a tool for direct analyses of biological tissues. The chapter is ended by a part devoted to data representation and management with a focus on free and open source software and data formats. The text is accompanied by a list of the cited literature.

Název v anglickém jazyce

Chapter 2 - Basis of mass spectrometry: Technical variants

Popis výsledku anglicky

Chapter 2, Basis of Mass Spectrometry: Technical Variants, provides a definition of mass spectrometry (MS) and describes the history of this analytical technique from the very beginning. Then it summarizes basic components of a mass spectrometer, from which namely mass analyzers are further described in detail together with their combinations in hybrid mass spectrometers. Such instruments are designed to perform tandem MS, which has been used in chemistry and biology to identify and quantify compounds. All possible modes of tandem MS are mentioned as well as common ion activation techniques for the fragmentation of gas-phase ions, which is essential and occurs between different stages of mass analysis. Next, separation techniques hyphenated with MS are discussed, namely gas and liquid chromatography (GC and LC, respectively) and capillary electrophoresis (CE). Hyphenated systems are nowadays almost indispensable for investigations of complex biological samples containing proteins, lipids, or metabolites. Mass spectrometry imaging (MSI) is presented as a tool for direct analyses of biological tissues. The chapter is ended by a part devoted to data representation and management with a focus on free and open source software and data formats. The text is accompanied by a list of the cited literature.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology

ISBN

978-0-12-814451-0

Počet stran výsledku

27

Strana od-do

19-45

Počet stran knihy

281

Název nakladatele

Elsevier Inc.

Místo vydání

Philadelphia

Kód UT WoS kapitoly

—