Identification and characterization of potential bioactive compounds from the leaves of Leucosidea sericea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73592232" target="_blank" >RIV/61989592:15310/18:73592232 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/18:00491769 RIV/61388963:_____/18:00491769

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0378874118300886" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378874118300886</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jep.2018.03.035" target="_blank" >10.1016/j.jep.2018.03.035</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification and characterization of potential bioactive compounds from the leaves of Leucosidea sericea

Popis výsledku v původním jazyce

Ethnopharmacological relevance: Leucosidea sericea is a southern African tree used for treating different diseases including microbial infections and inflammatory-related conditions. Aim of the study: To isolate and identify the chemicals in Leucosidea sericea which possibly account for the diverse therapeutic effects of the species. Materials and methods: Leaf material was extracted using 20% methanol and subsequently partitioned with different solvents (hexane, dichloromethane, ethyl acetate and butanol). Resultant fractions were subjected to bioactive (antimicrobial)-guided isolation and the structural elucidation was conducted using NMR (1D and 2D) spectroscopic methods. Given the broad uses of Leucosidea sericea in traditional medicine, the extract, fractions and isolated compounds were evaluated in five (5) biological assays in vitro (antimicrobial, antioxidant, acetylcholinesterase (AChE) and anti-inflammatory inhibition as well as cytotoxicity effect). Results: As the most active fractions, from ethyl acetate yielded 5,7-dihydroxychromone (1); 1-hydroxy-2-oxopomolic acid (2); 3,5,7,3&apos;,4&apos;-pentahydroxyflavone (3) and Tiliroside (4). For the first time, these four (4) compounds were isolated from leaves of Leucosidea sericea. These aforementioned compounds demonstrated broad-spectrum antibacterial activity (1.95-125 mu g/mL) and noteworthy antifungal (3.9-250 mu g/mL) potential. In addition to its noteworthy antimicrobial activity, compound 3 also demonstrated significant antioxidant (EC50 = 14 mu g/mL in DPPH assay) and anti-inflammatory (inhibited the level of ELAM by approximately 36% and decreased also the viability of endothelial cells) activities in vitro. Overall, AChE inhibition activity and cytotoxic response was generally weak for the extracts, fractions and isolated compounds. Conclusion: The pool of chemicals in Leucosidea sericea were enriched with the isolation and identification of four (4) compounds obtained from the leaf extract. Among these compounds, the significant antimicrobial activity of compound 3 provides strong evidence that support the use of Leucosidea sericea for microbial-related infections in folk medicine.

Název v anglickém jazyce

Identification and characterization of potential bioactive compounds from the leaves of Leucosidea sericea

Popis výsledku anglicky

Ethnopharmacological relevance: Leucosidea sericea is a southern African tree used for treating different diseases including microbial infections and inflammatory-related conditions. Aim of the study: To isolate and identify the chemicals in Leucosidea sericea which possibly account for the diverse therapeutic effects of the species. Materials and methods: Leaf material was extracted using 20% methanol and subsequently partitioned with different solvents (hexane, dichloromethane, ethyl acetate and butanol). Resultant fractions were subjected to bioactive (antimicrobial)-guided isolation and the structural elucidation was conducted using NMR (1D and 2D) spectroscopic methods. Given the broad uses of Leucosidea sericea in traditional medicine, the extract, fractions and isolated compounds were evaluated in five (5) biological assays in vitro (antimicrobial, antioxidant, acetylcholinesterase (AChE) and anti-inflammatory inhibition as well as cytotoxicity effect). Results: As the most active fractions, from ethyl acetate yielded 5,7-dihydroxychromone (1); 1-hydroxy-2-oxopomolic acid (2); 3,5,7,3&apos;,4&apos;-pentahydroxyflavone (3) and Tiliroside (4). For the first time, these four (4) compounds were isolated from leaves of Leucosidea sericea. These aforementioned compounds demonstrated broad-spectrum antibacterial activity (1.95-125 mu g/mL) and noteworthy antifungal (3.9-250 mu g/mL) potential. In addition to its noteworthy antimicrobial activity, compound 3 also demonstrated significant antioxidant (EC50 = 14 mu g/mL in DPPH assay) and anti-inflammatory (inhibited the level of ELAM by approximately 36% and decreased also the viability of endothelial cells) activities in vitro. Overall, AChE inhibition activity and cytotoxic response was generally weak for the extracts, fractions and isolated compounds. Conclusion: The pool of chemicals in Leucosidea sericea were enriched with the isolation and identification of four (4) compounds obtained from the leaf extract. Among these compounds, the significant antimicrobial activity of compound 3 provides strong evidence that support the use of Leucosidea sericea for microbial-related infections in folk medicine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF ETHNOPHARMACOLOGY

ISSN

0378-8741

e-ISSN

—

Svazek periodika

220

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

169-176

Kód UT WoS článku

000435062900017

EID výsledku v databázi Scopus

2-s2.0-85045216937