Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73608446" target="_blank" >RIV/61989592:15310/21:73608446 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2227-9040/9/6/119/htm" target="_blank" >https://www.mdpi.com/2227-9040/9/6/119/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/chemosensors9060119" target="_blank" >10.3390/chemosensors9060119</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection

Popis výsledku v původním jazyce

A new concept for simultaneous detection of two model proteases based on immobilized fluorescently labelled peptides was developed and evaluated. Each probe was composed of a carrier modified by poly(ethylene glycol) (PEG) chains, a specifically cleavable linker, and a fluorescent dye incorporated in the peptide tail. Based on a single excitation-double emission fluorescence response of liberated fluorophores caused by enzymatic cleavage, the presence of a single or both proteases in a mixture was unambiguously determined in an experimentally established concentration range. Among the tested solid supports, Rink Amide PEGA resin was recognized as the most suitable choice from the perspective of on-resin enzyme assays.

Název v anglickém jazyce

Immobilized Fluorescent Probes for Simultaneous Multiple Protease Detection

Popis výsledku anglicky

A new concept for simultaneous detection of two model proteases based on immobilized fluorescently labelled peptides was developed and evaluated. Each probe was composed of a carrier modified by poly(ethylene glycol) (PEG) chains, a specifically cleavable linker, and a fluorescent dye incorporated in the peptide tail. Based on a single excitation-double emission fluorescence response of liberated fluorophores caused by enzymatic cleavage, the presence of a single or both proteases in a mixture was unambiguously determined in an experimentally established concentration range. Among the tested solid supports, Rink Amide PEGA resin was recognized as the most suitable choice from the perspective of on-resin enzyme assays.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescenční sondy aktivované hydrolasami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosensors

ISSN

2227-9040

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

"119-1"-"119-13"

Kód UT WoS článku

000665373600001

EID výsledku v databázi Scopus

2-s2.0-85107462035