Fluorescence-Based On-Resin Detection of ThreeModel Proteases

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73609992" target="_blank" >RIV/61989592:15310/21:73609992 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2227-9040/9/12/359/htm" target="_blank" >https://www.mdpi.com/2227-9040/9/12/359/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/chemosensors9120359" target="_blank" >10.3390/chemosensors9120359</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescence-Based On-Resin Detection of ThreeModel Proteases

Popis výsledku v původním jazyce

A new approach to on-resin detection of three model proteases (trypsin, chymotrypsin, and thrombin) has been developed, while at the same time already described methodology for simultaneous detection of two enzymes (trypsin and chymotrypsin) has been additionally generalized. Appropriate immobilized substrates, comprising specifically cleavable peptide sequences capped with fluorescent dyes, have been synthesized on Rink Amide PEGA resin or Amino PEGA resin modified with backbone amide linker (BAL). Resulting solid support-bound probes were then dispersed into Tris-HCl buffer solution (pH = 8.0) and subjected to enzymatic cleavage. Liberated fluorophores have been tracked by fluorescence measuring. The competitive activities of studied proteases towards the thrombin probe have been efficiently limited and controlled by employing a Bowman-Birk inhibitor into a system.

Název v anglickém jazyce

Fluorescence-Based On-Resin Detection of ThreeModel Proteases

Popis výsledku anglicky

A new approach to on-resin detection of three model proteases (trypsin, chymotrypsin, and thrombin) has been developed, while at the same time already described methodology for simultaneous detection of two enzymes (trypsin and chymotrypsin) has been additionally generalized. Appropriate immobilized substrates, comprising specifically cleavable peptide sequences capped with fluorescent dyes, have been synthesized on Rink Amide PEGA resin or Amino PEGA resin modified with backbone amide linker (BAL). Resulting solid support-bound probes were then dispersed into Tris-HCl buffer solution (pH = 8.0) and subjected to enzymatic cleavage. Liberated fluorophores have been tracked by fluorescence measuring. The competitive activities of studied proteases towards the thrombin probe have been efficiently limited and controlled by employing a Bowman-Birk inhibitor into a system.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescenční sondy aktivované hydrolasami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosensors

ISSN

2227-9040

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

"359-1"-"359-11"

Kód UT WoS článku

000743064700001

EID výsledku v databázi Scopus

2-s2.0-85121827980