Unified chromatography - Mass spectrometry as a versatile tool for determination of food dyes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73609478" target="_blank" >RIV/61989592:15310/21:73609478 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0003267021002270" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0003267021002270</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2021.338401" target="_blank" >10.1016/j.aca.2021.338401</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Unified chromatography - Mass spectrometry as a versatile tool for determination of food dyes

Popis výsledku v původním jazyce

Unified chromatography with mass spectrometric detection was assessed for determination of food dyes. Nineteen substances representing azo, triphenylmethane, xanthone, indigoid, quinoline and polyene classes covering an unprecedented range from nonpolar beta-Carotene (logD 13.6) to ionic Tartrazine (logD-7.5) were analyzed simultaneously. The dyes were separated in a single experimental run by an 18-min gradient elution from 98% CO2 to 100% aqueous-methanolic modifier on a diol column. Isomeric substances were resolved, and Isatin Sulfonic acid was detected as a degradation product of Indigo Carmine. Mobile phase properties reproducibly changed from supercritical to liquid state ensuring stable retention times (inter-day RSD&lt;0.5%). Quantitative analysis of sports drinks after straightforward 10- or 25-fold dilution with dimethyl sulfoxide confirmed the method applicability to real-life samples. Suffi-cient limits of detection (typically 0.025 mg L-1 in processed samples, equivalent to 0.25 mg L-1 in drink) and a wide linear range (typically 0.5-50 mg L-1 or 1.3-125 mg L-1 in drink for 10x or 25x dilution, respectively) were demonstrated during validation. A comparison of method performance with competitive liquid chromatography procedures is also provided. Unified chromatography is a promising tool for comprehensive multiclass analysis of dyes in the context of food safety.

Název v anglickém jazyce

Unified chromatography - Mass spectrometry as a versatile tool for determination of food dyes

Popis výsledku anglicky

Unified chromatography with mass spectrometric detection was assessed for determination of food dyes. Nineteen substances representing azo, triphenylmethane, xanthone, indigoid, quinoline and polyene classes covering an unprecedented range from nonpolar beta-Carotene (logD 13.6) to ionic Tartrazine (logD-7.5) were analyzed simultaneously. The dyes were separated in a single experimental run by an 18-min gradient elution from 98% CO2 to 100% aqueous-methanolic modifier on a diol column. Isomeric substances were resolved, and Isatin Sulfonic acid was detected as a degradation product of Indigo Carmine. Mobile phase properties reproducibly changed from supercritical to liquid state ensuring stable retention times (inter-day RSD&lt;0.5%). Quantitative analysis of sports drinks after straightforward 10- or 25-fold dilution with dimethyl sulfoxide confirmed the method applicability to real-life samples. Suffi-cient limits of detection (typically 0.025 mg L-1 in processed samples, equivalent to 0.25 mg L-1 in drink) and a wide linear range (typically 0.5-50 mg L-1 or 1.3-125 mg L-1 in drink for 10x or 25x dilution, respectively) were demonstrated during validation. A comparison of method performance with competitive liquid chromatography procedures is also provided. Unified chromatography is a promising tool for comprehensive multiclass analysis of dyes in the context of food safety.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ANALYTICA CHIMICA ACTA

ISSN

0003-2670

e-ISSN

—

Svazek periodika

1157

Číslo periodika v rámci svazku

MAY

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

"338401-1"-"338401-11"

Kód UT WoS článku

000637650300002

EID výsledku v databázi Scopus

2-s2.0-85102755027