Sub-1 min separation in sequential injection chromatography for determination of synthetic water-soluble dyes in pharmaceutical formulation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364762" target="_blank" >RIV/00216208:11160/17:10364762 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0731708517304041" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0731708517304041</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jpba.2017.05.036" target="_blank" >10.1016/j.jpba.2017.05.036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sub-1 min separation in sequential injection chromatography for determination of synthetic water-soluble dyes in pharmaceutical formulation

Popis výsledku v původním jazyce

Sequential Injection Chromatography (SIC) evolved from fast and automated non-separation Sequential Injection Analysis (SIA) into chromatographic separation method for multi-element analysis. However, the speed of the measurement (sample throughput) is due to chromatography significantly reduced. In this paper, a sub-1 min separation using medium polar cyano monolithic column (5 mm x 4.6 mm) resulted in fast and green separation with sample throughput comparable with non-separation flow methods The separation of three synthetic water-soluble dyes (sunset yellow FCF, carmoisine and green S) was in a gradient elution mode (0.02% ammonium acetate, pH 6.7- water) with flow rate of 3.0 mL min(-1) corresponding with sample throughput of 30 h(-1). Spectrophotometric detection wavelengths were set to 480, 516 and 630 nm and 10 Hz data collection rate. The performance of the separation was described and discussed (peak capacities 3.48-7.67, peak symmetries 1.72-1.84 and resolutions 1.42-1.88). The method was represented by validation parameters: LODs of 0.15-0.35 mg L-1, LOQs of 0.50-1.25 mg L-1, calibration ranges 0.50-150.00 mg L-1 (r &gt; 0.998) and repeatability at 10.0 mg L-1 of RSD &lt;= 0.98% (n = 6). The method was used for determination of the dyes in &quot;forest berries&quot; colored pharmaceutical cough-cold formulation. The sample matrix - pharmaceuticals and excipients were not interfering with vis determination because of no retention in the separation column and colorless nature. The results proved the concept of fast and green chromatography approach using very short medium polar monolithic column in SIC.

Název v anglickém jazyce

Sub-1 min separation in sequential injection chromatography for determination of synthetic water-soluble dyes in pharmaceutical formulation

Popis výsledku anglicky

Sequential Injection Chromatography (SIC) evolved from fast and automated non-separation Sequential Injection Analysis (SIA) into chromatographic separation method for multi-element analysis. However, the speed of the measurement (sample throughput) is due to chromatography significantly reduced. In this paper, a sub-1 min separation using medium polar cyano monolithic column (5 mm x 4.6 mm) resulted in fast and green separation with sample throughput comparable with non-separation flow methods The separation of three synthetic water-soluble dyes (sunset yellow FCF, carmoisine and green S) was in a gradient elution mode (0.02% ammonium acetate, pH 6.7- water) with flow rate of 3.0 mL min(-1) corresponding with sample throughput of 30 h(-1). Spectrophotometric detection wavelengths were set to 480, 516 and 630 nm and 10 Hz data collection rate. The performance of the separation was described and discussed (peak capacities 3.48-7.67, peak symmetries 1.72-1.84 and resolutions 1.42-1.88). The method was represented by validation parameters: LODs of 0.15-0.35 mg L-1, LOQs of 0.50-1.25 mg L-1, calibration ranges 0.50-150.00 mg L-1 (r &gt; 0.998) and repeatability at 10.0 mg L-1 of RSD &lt;= 0.98% (n = 6). The method was used for determination of the dyes in &quot;forest berries&quot; colored pharmaceutical cough-cold formulation. The sample matrix - pharmaceuticals and excipients were not interfering with vis determination because of no retention in the separation column and colorless nature. The results proved the concept of fast and green chromatography approach using very short medium polar monolithic column in SIC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/GA15-10781S" target="_blank" >GA15-10781S: On-line spojení automatizovaných extrakčních procesů s kapalinovou chromatografií pro kompletní analýzu vzorku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Pharmaceutical and Biomedical Analysis

ISSN

0731-7085

e-ISSN

—

Svazek periodika

143

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

123-129

Kód UT WoS článku

000405537300017

EID výsledku v databázi Scopus

2-s2.0-85019984072