Fast separation of red colorants in beverages using cyano monolithic column in Sequential Injection Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10364766" target="_blank" >RIV/00216208:11160/17:10364766 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0026265X16302971" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0026265X16302971</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2016.10.022" target="_blank" >10.1016/j.microc.2016.10.022</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fast separation of red colorants in beverages using cyano monolithic column in Sequential Injection Chromatography

Popis výsledku v původním jazyce

Flexible medium-pressure Sequential Injection Chromatography (SIC) flow manifold was used for development of a fast green chromatography method. The article presents novel use of a cyano monolithic column (Merck Chromolith 50 mm x 4.6 mm) for a separation of three water soluble colorants (carmoisine, Ponceau 4R and red 2G), aiming the extension of the SIC separation capabilities for polar analytes. The separation was performed in a gradient elution mode with changing concentration (0.05-0.005% v/v) of ammonium phosphate at pH 6.0 as a mobile phase. No organic modifier was used in mobile phase, thus the green chromatography rules were fulfilled. The vis spectrophotometric detection was set at 500 nm. High performance of the separation with the cyano monolithic column was described and discussed (peak capacities 6.67-10.94, peak symmetries 1.11-1.50, resolutions &gt;= 2.78 and time for one analysis 3.15 min). The method was represented by analytical parameters - LODs of 0.45 mg L-1, a LOQs of 1.5 mg L-1 and calibration range of 1.5-60.0 mg L-1 (r &gt; 0.997) for all analytes, and a method repeatability at 10 mg L-1 of RSD &lt;= 2.69% (n = 5) and system precision of RSD &lt; 1.55% (n = 6). The developed method was used for determination of carmoisine and Ponceau 4R in red coloured beverages with red 2G as an internal standard. The results confirmed benefits of extended chromatographic selectivity of cyano monolithic column in the SIC.

Název v anglickém jazyce

Fast separation of red colorants in beverages using cyano monolithic column in Sequential Injection Chromatography

Popis výsledku anglicky

Flexible medium-pressure Sequential Injection Chromatography (SIC) flow manifold was used for development of a fast green chromatography method. The article presents novel use of a cyano monolithic column (Merck Chromolith 50 mm x 4.6 mm) for a separation of three water soluble colorants (carmoisine, Ponceau 4R and red 2G), aiming the extension of the SIC separation capabilities for polar analytes. The separation was performed in a gradient elution mode with changing concentration (0.05-0.005% v/v) of ammonium phosphate at pH 6.0 as a mobile phase. No organic modifier was used in mobile phase, thus the green chromatography rules were fulfilled. The vis spectrophotometric detection was set at 500 nm. High performance of the separation with the cyano monolithic column was described and discussed (peak capacities 6.67-10.94, peak symmetries 1.11-1.50, resolutions &gt;= 2.78 and time for one analysis 3.15 min). The method was represented by analytical parameters - LODs of 0.45 mg L-1, a LOQs of 1.5 mg L-1 and calibration range of 1.5-60.0 mg L-1 (r &gt; 0.997) for all analytes, and a method repeatability at 10 mg L-1 of RSD &lt;= 2.69% (n = 5) and system precision of RSD &lt; 1.55% (n = 6). The developed method was used for determination of carmoisine and Ponceau 4R in red coloured beverages with red 2G as an internal standard. The results confirmed benefits of extended chromatographic selectivity of cyano monolithic column in the SIC.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/GA15-10781S" target="_blank" >GA15-10781S: On-line spojení automatizovaných extrakčních procesů s kapalinovou chromatografií pro kompletní analýzu vzorku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchemical Journal

ISSN

0026-265X

e-ISSN

—

Svazek periodika

130

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

384-389

Kód UT WoS článku

000390072400054

EID výsledku v databázi Scopus

2-s2.0-84994017856