New generation of sequential injection chromatography: Great enhancement of capabilities of separation using flow analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10399617" target="_blank" >RIV/00216208:11160/19:10399617 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=lO669h3goe" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=lO669h3goe</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2019.05.108" target="_blank" >10.1016/j.talanta.2019.05.108</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New generation of sequential injection chromatography: Great enhancement of capabilities of separation using flow analysis

Popis výsledku v původním jazyce

Since its inception, sequential injection chromatography (SIC) has evolved through several stages. Key moments including introduction of the novel technique combining sequential injection analysis and monolithic column, the first generation of commercial SIC system employing robust pump, the utilization of columns packed with fused-core particles, the on-line hyphenation of extraction and separation steps in SIC, are now followed by the second generation of commercial SIC system employing stainless steel syringe pump and parts optimized for chromatographic separation. The key developments always mean acceleration of the evolution by opening new avenues and reduction of compromises in automated analytical methods based on the flow analysis. The updates, new features, and prospects of the novel instrument are described and discussed on perspective of the method developed for extraction and separation of selected phenolic acids (gallic, protocatechuic, caffeic, p-coumaric and ferulic). The method hyphenates miniaturized on-line solid phase extraction using strong anion exchange sorbent in commercial cartridge for HPLC (20 x 1 mm) and liquid chromatography using chromatographic column (C18 50 x 4.6 mm, 5 mu m particles) packed with fused-core particles in the SIC manifold. The separation in gradient mode used acetonitrile: aqueous formic acid pH 2.0 mobile phase and spectrophotometric detection at 270, 300, and 320 nm. Injected sample volumes were 200 and 500 mu L. The performance of the extraction step was characterized by the recovery 94.0-107.8%, enrichment factors about 20 or 50, and the separation by peak capacities 13-34, peak symmetries 1.17-1.64, and resolutions 0.82-3.75). While using a sample volume of 200 mu L, our method was characterized by the following validation parameters: LODs of 0.0075-0.03 mg L-1, LOQs of 0.025-0.10 mg L-1, calibration ranges 0.025-2.50 mg L-1 (r &gt; 0.999), repeatability of signal at 0.50 mg L-1 of RSD &lt;= 1.46% (n = 6), and overall time of analysis 7.1 min. The results including pilot analysis of white and red wines demonstrated the capability of novel SIC instrument to enable fast, selective, and sensitive analysis.

Název v anglickém jazyce

New generation of sequential injection chromatography: Great enhancement of capabilities of separation using flow analysis

Popis výsledku anglicky

Since its inception, sequential injection chromatography (SIC) has evolved through several stages. Key moments including introduction of the novel technique combining sequential injection analysis and monolithic column, the first generation of commercial SIC system employing robust pump, the utilization of columns packed with fused-core particles, the on-line hyphenation of extraction and separation steps in SIC, are now followed by the second generation of commercial SIC system employing stainless steel syringe pump and parts optimized for chromatographic separation. The key developments always mean acceleration of the evolution by opening new avenues and reduction of compromises in automated analytical methods based on the flow analysis. The updates, new features, and prospects of the novel instrument are described and discussed on perspective of the method developed for extraction and separation of selected phenolic acids (gallic, protocatechuic, caffeic, p-coumaric and ferulic). The method hyphenates miniaturized on-line solid phase extraction using strong anion exchange sorbent in commercial cartridge for HPLC (20 x 1 mm) and liquid chromatography using chromatographic column (C18 50 x 4.6 mm, 5 mu m particles) packed with fused-core particles in the SIC manifold. The separation in gradient mode used acetonitrile: aqueous formic acid pH 2.0 mobile phase and spectrophotometric detection at 270, 300, and 320 nm. Injected sample volumes were 200 and 500 mu L. The performance of the extraction step was characterized by the recovery 94.0-107.8%, enrichment factors about 20 or 50, and the separation by peak capacities 13-34, peak symmetries 1.17-1.64, and resolutions 0.82-3.75). While using a sample volume of 200 mu L, our method was characterized by the following validation parameters: LODs of 0.0075-0.03 mg L-1, LOQs of 0.025-0.10 mg L-1, calibration ranges 0.025-2.50 mg L-1 (r &gt; 0.999), repeatability of signal at 0.50 mg L-1 of RSD &lt;= 1.46% (n = 6), and overall time of analysis 7.1 min. The results including pilot analysis of white and red wines demonstrated the capability of novel SIC instrument to enable fast, selective, and sensitive analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/EF15_003%2F0000465" target="_blank" >EF15_003/0000465: Vytvoření expertního týmu pro pokročilý výzkum v separačních vědách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

204

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

272-277

Kód UT WoS článku

000480664200032

EID výsledku v databázi Scopus

2-s2.0-85066834923