New Generation of Arabidopsis thaliana Cytokinin Oxidase/Dehydrogenase Inhibitors Affect Shoot/Root Growth and Seed Yield

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73610836" target="_blank" >RIV/61989592:15310/21:73610836 - isvavai.cz</a>

Výsledek na webu

<a href="https://obd.upol.cz/id_publ/333190723" target="_blank" >https://obd.upol.cz/id_publ/333190723</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-981-15-9046-7_21" target="_blank" >10.1007/978-981-15-9046-7_21</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New Generation of Arabidopsis thaliana Cytokinin Oxidase/Dehydrogenase Inhibitors Affect Shoot/Root Growth and Seed Yield

Popis výsledku v původním jazyce

This chapter describes the preparation of a new group of cytokinin oxidase/dehydrogenase (CKX) enzyme inhibitors usable for different agricultural applications. Recently developed CKX inhibitors, 2-chloro-6-(3-methoxyphenylamino)purine (INCYDE) and 2-fluoro-6-(3-methoxyphenylamino)purine (INCYDE-F) were modified by the tetrahydropyran-2-yl (THP) group at the N9-position to obtain 2-chloro-6-(3-methoxyanilino)-9-(tetrahydropyran-2-yl)purine (INCYDE-THP) and 2-fluoro-6-(3-methoxyanilino)-9-(tetrahydropyran-2-yl)purine (INCYDE-F-THP), respectively. These compounds were used to inhibit CKX from Arabidopsis thaliana (AtCKX2). Further, their biological activity was investigated in a receptor binding assay where its ability to activate cytokinin receptors was assessed. Activation of the cytokinin signalling pathway was determined by ARR5/GUS reporter gene assay. All the assays showed that INCYDE-THP is a medium AtCKX2 inhibitor, average cytokinin receptor agonist as well as inducer of ARR5 gene expression (a member of the A-type response regulator family classified as cytokinin primary response genes). INCYDE-F-THP was, however, found to be almost inactive. Their biological activity was also compared in three different bioassays based on the stimulation of tobacco callus growth, the retention of chlorophyll in excised wheat leaves and the dark induction of betacyanin synthesis in Amaranthus cotyledons. The two compounds were, however, only mildly active (&lt;10% of BAP) in tobacco callus and Amaranthus bioassay and almost inactive in the senescence test. Finally, in vitro and in vivo experiments carried out with INCYDE-THP on different plant species showed positive effects on plant growth and development. In summary, new perspective inhibitors of AtCKX2 were found. These positive effects are also reflected in increased yields of some crops, such as wheat, corn and oilseed rape, and some vegetables, especially when applied by watering. In conclusion, INCYDE-THP is a promising candidate for plant irrigation and seed coating that could contribute as an agrochemical approach to the growth of shoot/root biomass than standard molecular biology methods.

Název v anglickém jazyce

New Generation of Arabidopsis thaliana Cytokinin Oxidase/Dehydrogenase Inhibitors Affect Shoot/Root Growth and Seed Yield

Popis výsledku anglicky

This chapter describes the preparation of a new group of cytokinin oxidase/dehydrogenase (CKX) enzyme inhibitors usable for different agricultural applications. Recently developed CKX inhibitors, 2-chloro-6-(3-methoxyphenylamino)purine (INCYDE) and 2-fluoro-6-(3-methoxyphenylamino)purine (INCYDE-F) were modified by the tetrahydropyran-2-yl (THP) group at the N9-position to obtain 2-chloro-6-(3-methoxyanilino)-9-(tetrahydropyran-2-yl)purine (INCYDE-THP) and 2-fluoro-6-(3-methoxyanilino)-9-(tetrahydropyran-2-yl)purine (INCYDE-F-THP), respectively. These compounds were used to inhibit CKX from Arabidopsis thaliana (AtCKX2). Further, their biological activity was investigated in a receptor binding assay where its ability to activate cytokinin receptors was assessed. Activation of the cytokinin signalling pathway was determined by ARR5/GUS reporter gene assay. All the assays showed that INCYDE-THP is a medium AtCKX2 inhibitor, average cytokinin receptor agonist as well as inducer of ARR5 gene expression (a member of the A-type response regulator family classified as cytokinin primary response genes). INCYDE-F-THP was, however, found to be almost inactive. Their biological activity was also compared in three different bioassays based on the stimulation of tobacco callus growth, the retention of chlorophyll in excised wheat leaves and the dark induction of betacyanin synthesis in Amaranthus cotyledons. The two compounds were, however, only mildly active (&lt;10% of BAP) in tobacco callus and Amaranthus bioassay and almost inactive in the senescence test. Finally, in vitro and in vivo experiments carried out with INCYDE-THP on different plant species showed positive effects on plant growth and development. In summary, new perspective inhibitors of AtCKX2 were found. These positive effects are also reflected in increased yields of some crops, such as wheat, corn and oilseed rape, and some vegetables, especially when applied by watering. In conclusion, INCYDE-THP is a promising candidate for plant irrigation and seed coating that could contribute as an agrochemical approach to the growth of shoot/root biomass than standard molecular biology methods.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture

ISBN

978-981-15-9045-0

Počet stran výsledku

24

Strana od-do

293-316

Počet stran knihy

339

Název nakladatele

Springer

Místo vydání

Singapore

Kód UT WoS kapitoly

—