Development of fluorescent dual-FRET probe for simultaneous detection of caspase-8 and caspase-9 activities and their relative quantification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73615731" target="_blank" >RIV/61989592:15310/22:73615731 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0045206822005570" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0045206822005570</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioorg.2022.106151" target="_blank" >10.1016/j.bioorg.2022.106151</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Development of fluorescent dual-FRET probe for simultaneous detection of caspase-8 and caspase-9 activities and their relative quantification

Popis výsledku v původním jazyce

A multi-FRET three-fluorophore probe containing coumarin, fluorescein and rhodamine B with two enzymati-cally cleavable linkers has been synthesized and optimized for the simultaneous activity detection and relative quantification of two proteases – caspase-8 and caspase-9. The probe designed as a ratiometric single-excitation triple-emission system shows specific change in fluorescence intensities upon enzymatic cleavage of individual linkers in model mixtures as well as in a cell lysate. The activation of caspase-8 and caspase-9 is responsible for initiation of extrinsic or intrinsic apoptotic pathway, respectively, and the probe was proposed as a single chemical tool which could help to decipher a mechanism of cell death induced by various stimuli. The main advantage of this probe is the simplicity of its preparation using conventional organic synthesis, easy application for measurement and evaluation of the results.

Název v anglickém jazyce

Development of fluorescent dual-FRET probe for simultaneous detection of caspase-8 and caspase-9 activities and their relative quantification

Popis výsledku anglicky

A multi-FRET three-fluorophore probe containing coumarin, fluorescein and rhodamine B with two enzymati-cally cleavable linkers has been synthesized and optimized for the simultaneous activity detection and relative quantification of two proteases – caspase-8 and caspase-9. The probe designed as a ratiometric single-excitation triple-emission system shows specific change in fluorescence intensities upon enzymatic cleavage of individual linkers in model mixtures as well as in a cell lysate. The activation of caspase-8 and caspase-9 is responsible for initiation of extrinsic or intrinsic apoptotic pathway, respectively, and the probe was proposed as a single chemical tool which could help to decipher a mechanism of cell death induced by various stimuli. The main advantage of this probe is the simplicity of its preparation using conventional organic synthesis, easy application for measurement and evaluation of the results.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescenční sondy aktivované hydrolasami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOORGANIC CHEMISTRY

ISSN

0045-2068

e-ISSN

1090-2120

Svazek periodika

129

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

"106151-1"-"106151-11"

Kód UT WoS článku

000888608800005

EID výsledku v databázi Scopus

2-s2.0-85139410601