Triple-FRET multi-purpose fluorescent probe for three-protease detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73615737" target="_blank" >RIV/61989592:15310/22:73615737 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlepdf/2022/ra/d2ra05125g" target="_blank" >https://pubs.rsc.org/en/content/articlepdf/2022/ra/d2ra05125g</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d2ra05125g" target="_blank" >10.1039/d2ra05125g</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Triple-FRET multi-purpose fluorescent probe for three-protease detection

Popis výsledku v původním jazyce

A new, robust and reliable methodology for three-protease screening in a single-enzyme mode has been developed and verified, employing a multi-purpose peptide probe with three selectively cleavable sites furnished with four fluorophores. A triple-FRET-based single-excitation quadruple-emission concept for unambiguous sensing of trypsin, chymotrypsin and caspase-8 in the lowest detectable concentrations of 0.5 ng mL(-1), 0.2 mu g mL(-1), and 2 U mL(-1), respectively, has been applied and graphically depicted. Then the developed 4-dye probe has been also studied from the perspective of simultaneous two-protease screening, which was found only partially feasible, primarily due to unselective chymotrypsin cleavage.

Název v anglickém jazyce

Triple-FRET multi-purpose fluorescent probe for three-protease detection

Popis výsledku anglicky

A new, robust and reliable methodology for three-protease screening in a single-enzyme mode has been developed and verified, employing a multi-purpose peptide probe with three selectively cleavable sites furnished with four fluorophores. A triple-FRET-based single-excitation quadruple-emission concept for unambiguous sensing of trypsin, chymotrypsin and caspase-8 in the lowest detectable concentrations of 0.5 ng mL(-1), 0.2 mu g mL(-1), and 2 U mL(-1), respectively, has been applied and graphically depicted. Then the developed 4-dye probe has been also studied from the perspective of simultaneous two-protease screening, which was found only partially feasible, primarily due to unselective chymotrypsin cleavage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GA19-23972S" target="_blank" >GA19-23972S: Multi-FRET fluorescenční sondy aktivované hydrolasami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

RSC Advances

ISSN

2046-2069

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

44

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

28780-28787

Kód UT WoS článku

000865527900001

EID výsledku v databázi Scopus

2-s2.0-85141595688