Effect of ion fluxes on regulating the light-induced transthylakoid electric potential difference.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73614824" target="_blank" >RIV/61989592:15310/23:73614824 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0981942822004880" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0981942822004880</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2022.10.028" target="_blank" >10.1016/j.plaphy.2022.10.028</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of ion fluxes on regulating the light-induced transthylakoid electric potential difference.

Popis výsledku v původním jazyce

The light-induced transthylakoid membrane potential (ΔΨ) can not only drive the ATP synthesis through the ATP-synthase in chloroplasts but serve as an essential modifier in the acclimation of photosynthesis to fluctuating light conditions. It has been manifested that during photosynthesis, the light-induced ΔΨ is responsive to multiple factors among which the ion channels/transporters (e.g., V–K+, VCCN1, and KEA3) are key to adjust the ion distribution on the two sides of the thylakoid membrane and hence shape the kinetics of ΔΨ. However, an in-depth mechanistic understanding of ion fluxes on adjusting the transthylakoid electric potentials is still unclear. This lack of a mechanistic understanding is due to the experimental difficulty of closely observing ion fluxes in vivo and also hacking the evolution of parameters in a highly intertwined photosynthetic network. In this work, a computer model was applied to investigate the roles of ion fluxes on adjusting transthylakoid electric potentials upon a temporal cycle of a period of high illumination followed by a dark-adapted phase. The computing data revealed that, firstly, upon illumination, the dissipation of the steady-ΔΨ by ∼10 mV is contributed from the V–K+-driven K+ flux whilst ∼8 mV of the steady-ΔΨ is dissipated by the VCCN1-pumped Cl− flux, but there were no appreciable KEA3-evoked variations on ΔΨ; secondly, on transition from high light to darkness, V–K+ and KEA3 are serving as major contributors whereas VCCN1 taking a counterbalancing part in shaping a standard trace of ECS (electrochromic shift), which commonly shows a sharp fall to a minimum before returning to the baseline in darkness. Besides, the functional consequences on components of ΔΨ adjusted by every particular ion channel/transporter were also explored. By employing the model, we bring evidence that particular thylakoid-harbored proteins, namely V–K+, VCCN1, and KEA3, function by distinct mechanisms in the dynamic adjustment of electric potential, which might be mainly importnat under fluctuating light conditions.

Název v anglickém jazyce

Effect of ion fluxes on regulating the light-induced transthylakoid electric potential difference.

Popis výsledku anglicky

The light-induced transthylakoid membrane potential (ΔΨ) can not only drive the ATP synthesis through the ATP-synthase in chloroplasts but serve as an essential modifier in the acclimation of photosynthesis to fluctuating light conditions. It has been manifested that during photosynthesis, the light-induced ΔΨ is responsive to multiple factors among which the ion channels/transporters (e.g., V–K+, VCCN1, and KEA3) are key to adjust the ion distribution on the two sides of the thylakoid membrane and hence shape the kinetics of ΔΨ. However, an in-depth mechanistic understanding of ion fluxes on adjusting the transthylakoid electric potentials is still unclear. This lack of a mechanistic understanding is due to the experimental difficulty of closely observing ion fluxes in vivo and also hacking the evolution of parameters in a highly intertwined photosynthetic network. In this work, a computer model was applied to investigate the roles of ion fluxes on adjusting transthylakoid electric potentials upon a temporal cycle of a period of high illumination followed by a dark-adapted phase. The computing data revealed that, firstly, upon illumination, the dissipation of the steady-ΔΨ by ∼10 mV is contributed from the V–K+-driven K+ flux whilst ∼8 mV of the steady-ΔΨ is dissipated by the VCCN1-pumped Cl− flux, but there were no appreciable KEA3-evoked variations on ΔΨ; secondly, on transition from high light to darkness, V–K+ and KEA3 are serving as major contributors whereas VCCN1 taking a counterbalancing part in shaping a standard trace of ECS (electrochromic shift), which commonly shows a sharp fall to a minimum before returning to the baseline in darkness. Besides, the functional consequences on components of ΔΨ adjusted by every particular ion channel/transporter were also explored. By employing the model, we bring evidence that particular thylakoid-harbored proteins, namely V–K+, VCCN1, and KEA3, function by distinct mechanisms in the dynamic adjustment of electric potential, which might be mainly importnat under fluctuating light conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT PHYSIOLOGY AND BIOCHEMISTRY

ISSN

0981-9428

e-ISSN

1873-2690

Svazek periodika

194

Číslo periodika v rámci svazku

JAN

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

10

Strana od-do

60-69

Kód UT WoS článku

000895736600006

EID výsledku v databázi Scopus

2-s2.0-85141750809