Molecular study of turnip mosaic virus population in the Czech Republic.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73621105" target="_blank" >RIV/61989592:15310/23:73621105 - isvavai.cz</a>

Výsledek na webu

<a href="https://pps.agriculturejournals.cz/pdfs/pps/2023/03/01.pdf" target="_blank" >https://pps.agriculturejournals.cz/pdfs/pps/2023/03/01.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/34/2023-PPS" target="_blank" >10.17221/34/2023-PPS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular study of turnip mosaic virus population in the Czech Republic.

Popis výsledku v původním jazyce

Turnip mosaic virus (TuMV) is the most important virus of brassica crops. In our study, we compare the genetic structure of two Czech TuMV populations sampled in the country&apos;s 25-year interval of virus presence. The 21 isolates, mainly infecting rutabaga and horseradish, were collected from four farms under organic production, and nearly complete genome sequences, 9 596-9 787 nt in length, were obtained using Sanger sequencing for all of them. The analysis of variability and polymorphism showed differences in genetic structure but the relative stability of both populations and moderate negative selection as a factor affecting the current TuMV population. The newly collected isolates are characterised by a relatively high frequency of intralineage recombinants; interlineage recombinants were not detected compared to the 25-year-old population. The phylogenetic analysis allowed the classification of all Czech isolates into world-B phylogroup, with the prevalence of isolates of subgroup B2. The spread of isolates belonging to the other phylogenetic groups posing higher phytopathological risk, which were present in the old population and some surrounding countries, was not found.

Název v anglickém jazyce

Molecular study of turnip mosaic virus population in the Czech Republic.

Popis výsledku anglicky

Turnip mosaic virus (TuMV) is the most important virus of brassica crops. In our study, we compare the genetic structure of two Czech TuMV populations sampled in the country&apos;s 25-year interval of virus presence. The 21 isolates, mainly infecting rutabaga and horseradish, were collected from four farms under organic production, and nearly complete genome sequences, 9 596-9 787 nt in length, were obtained using Sanger sequencing for all of them. The analysis of variability and polymorphism showed differences in genetic structure but the relative stability of both populations and moderate negative selection as a factor affecting the current TuMV population. The newly collected isolates are characterised by a relatively high frequency of intralineage recombinants; interlineage recombinants were not detected compared to the 25-year-old population. The phylogenetic analysis allowed the classification of all Czech isolates into world-B phylogroup, with the prevalence of isolates of subgroup B2. The spread of isolates belonging to the other phylogenetic groups posing higher phytopathological risk, which were present in the old population and some surrounding countries, was not found.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QK1910070" target="_blank" >QK1910070: Využití biotechnologických metod a netradičních genetických zdrojů k charakterizaci a tvorbě uniformních linií brukvovité zeleniny se specifickými parametry kvality, výnosu a rezistence k významným chorobám</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT PROTECTION SCIENCE

ISSN

1212-2580

e-ISSN

1805-9341

Svazek periodika

59

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

24

Strana od-do

209-232

Kód UT WoS článku

001098161200001

EID výsledku v databázi Scopus

2-s2.0-85172877609