A novel device for the determination of liposome/water partition coefficients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F24%3A73627460" target="_blank" >RIV/61989592:15310/24:73627460 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0039914023011852" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914023011852</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2023.125434" target="_blank" >10.1016/j.talanta.2023.125434</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel device for the determination of liposome/water partition coefficients

Popis výsledku v původním jazyce

A novel, cheap and easy-to-construct device and a simple method for partition coefficient determination in liposome/water system based on modified equilibrium dialysis have been developed. The device consists of two vials separated by a semi-permeable membrane, through which the free form of a low molecular weight sub-stance is transported by shaking assisted diffusion. Five test substances, eugenol, carvacrol, thymol, 4-hydroxy-benzyl alcohol (4-HBA) and butylparaben were analyzed after equilibration in aqueous phase by three methods, HPLC-UV, GC-MS and DPV with comparable results. This shows the possibility of using the proposed method in any laboratory with any equipment capable of analyzing the substance under study. The liposome/water partition coefficients (log Pl/w) determined for eugenol (2.39), thymol (2.83), carvacrol (2.78) and butylparaben (3.30) are consistent with previously published data. A strong effect of NaCl on the liposome/water partition coefficient was observed. The value of log Pl/w = 1.06 determined for 4-HBA in the presence of 0.15 mol L-1 NaCl in the partitioning liposomal system was considerably lower than in the absence of the salt (log Pl/w = 2.06). The developed method was used to determine the partition coefficient of morphine in liposome/water system without NaCl (log Pl/w = 2.65) under given conditions.

Název v anglickém jazyce

A novel device for the determination of liposome/water partition coefficients

Popis výsledku anglicky

A novel, cheap and easy-to-construct device and a simple method for partition coefficient determination in liposome/water system based on modified equilibrium dialysis have been developed. The device consists of two vials separated by a semi-permeable membrane, through which the free form of a low molecular weight sub-stance is transported by shaking assisted diffusion. Five test substances, eugenol, carvacrol, thymol, 4-hydroxy-benzyl alcohol (4-HBA) and butylparaben were analyzed after equilibration in aqueous phase by three methods, HPLC-UV, GC-MS and DPV with comparable results. This shows the possibility of using the proposed method in any laboratory with any equipment capable of analyzing the substance under study. The liposome/water partition coefficients (log Pl/w) determined for eugenol (2.39), thymol (2.83), carvacrol (2.78) and butylparaben (3.30) are consistent with previously published data. A strong effect of NaCl on the liposome/water partition coefficient was observed. The value of log Pl/w = 1.06 determined for 4-HBA in the presence of 0.15 mol L-1 NaCl in the partitioning liposomal system was considerably lower than in the absence of the salt (log Pl/w = 2.06). The developed method was used to determine the partition coefficient of morphine in liposome/water system without NaCl (log Pl/w = 2.65) under given conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-07350S" target="_blank" >GA20-07350S: Elektrochemické a analytické aspekty transportu návykových a psychotropních látek přes modelové biologické bariery</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TALANTA

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

269

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

"125434-1"-"125434-8"

Kód UT WoS článku

001165956200001

EID výsledku v databázi Scopus

2-s2.0-85181583919