Chemiexcitation in ex vivo porcine skin model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73632998" target="_blank" >RIV/61989592:15310/25:73632998 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/pcmr.70060" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/pcmr.70060</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/pcmr.70060" target="_blank" >10.1111/pcmr.70060</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chemiexcitation in ex vivo porcine skin model

Popis výsledku v původním jazyce

Chemiexcitation, the formation of electronically excited states via oxidative chemical reactions, has emerged as a potentially important contributor to skin photobiology beyond direct damage caused by ultraviolet (UV) radiation. This study investigates the hypothesis that UV radiation induces chemiexcitation in skin through the formation of triplet excited carbonyls, which may transfer energy to melanin and contribute to oxidative stress even after the termination of UV exposure. Using porcine skin as a model, we demonstrate that UV exposure leads to lipid peroxidation and the subsequent formation of organic radicals, including carbon-centered (alkyl) and oxygen-centered (peroxyl and alkoxyl) species, as detected by EPR spin-trapping spectroscopy. HPLC-MS analysis revealed that short-chain carbonyl compounds, such as formaldehyde and acetaldehyde, are the predominant electronically excited species in direct chemiexcitation. These triplet carbonyls can transfer their excitation energy to melanin through photon emission (radiative transfer) or direct electron exchange (non-radiative transfer), forming melanin-based secondary excited states via indirect chemiexcitation. These findings highlight a novel, light-independent mechanism of post-UV exposure oxidative damage in the skin and suggest a possible role for chemiexcitation in processes such as photoaging and photocarcinogenesis.

Název v anglickém jazyce

Chemiexcitation in ex vivo porcine skin model

Popis výsledku anglicky

Chemiexcitation, the formation of electronically excited states via oxidative chemical reactions, has emerged as a potentially important contributor to skin photobiology beyond direct damage caused by ultraviolet (UV) radiation. This study investigates the hypothesis that UV radiation induces chemiexcitation in skin through the formation of triplet excited carbonyls, which may transfer energy to melanin and contribute to oxidative stress even after the termination of UV exposure. Using porcine skin as a model, we demonstrate that UV exposure leads to lipid peroxidation and the subsequent formation of organic radicals, including carbon-centered (alkyl) and oxygen-centered (peroxyl and alkoxyl) species, as detected by EPR spin-trapping spectroscopy. HPLC-MS analysis revealed that short-chain carbonyl compounds, such as formaldehyde and acetaldehyde, are the predominant electronically excited species in direct chemiexcitation. These triplet carbonyls can transfer their excitation energy to melanin through photon emission (radiative transfer) or direct electron exchange (non-radiative transfer), forming melanin-based secondary excited states via indirect chemiexcitation. These findings highlight a novel, light-independent mechanism of post-UV exposure oxidative damage in the skin and suggest a possible role for chemiexcitation in processes such as photoaging and photocarcinogenesis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Pigment Cell and Melanoma Research

ISSN

1755-1471

e-ISSN

1755-148X

Svazek periodika

38

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

18

Strana od-do

"e70060-1"-"e70060-18"

Kód UT WoS článku

001626842500007

EID výsledku v databázi Scopus

2-s2.0-105018655819