Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73604523" target="_blank" >RIV/61989592:15640/21:73604523 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/21:73604523

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S187167842030193X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S187167842030193X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.nbt.2020.11.003" target="_blank" >10.1016/j.nbt.2020.11.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production

Popis výsledku v původním jazyce

The parasitic fungus Claviceps purpurea has been used for decades by the pharmaceutical industry as a valuable producer of ergot alkaloids. As the biosynthetic pathway of ergot alkaloids involves a common precursor L-tryptophan, targeted genetic modification of the related genes may improve production yield. In this work, the S76L mutated version of the trpE gene encoding anthranilate synthase was constitutively overexpressed in the fungus with the aim of overcoming feedback inhibition of the native enzyme by an excess of tryptophan. In another approach, the dmaW gene encoding dimethylallyltryptophan synthase, which produces a key intermediate for the biosynthesis of ergot alkaloids, was also constitutively overexpressed. Each of the above manipulations led to a significant increase (up to 7-fold) in the production of ergot alkaloids in submerged cultures.

Název v anglickém jazyce

Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production

Popis výsledku anglicky

The parasitic fungus Claviceps purpurea has been used for decades by the pharmaceutical industry as a valuable producer of ergot alkaloids. As the biosynthetic pathway of ergot alkaloids involves a common precursor L-tryptophan, targeted genetic modification of the related genes may improve production yield. In this work, the S76L mutated version of the trpE gene encoding anthranilate synthase was constitutively overexpressed in the fungus with the aim of overcoming feedback inhibition of the native enzyme by an excess of tryptophan. In another approach, the dmaW gene encoding dimethylallyltryptophan synthase, which produces a key intermediate for the biosynthesis of ergot alkaloids, was also constitutively overexpressed. Each of the above manipulations led to a significant increase (up to 7-fold) in the production of ergot alkaloids in submerged cultures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Biotechnology

ISSN

1871-6784

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

69-79

Kód UT WoS článku

000606593400009

EID výsledku v databázi Scopus

2-s2.0-85096827896