Multifunctional 3D Hierarchical Bioactive Green Carbon-Based Nanocomposites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73612400" target="_blank" >RIV/61989592:15640/21:73612400 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acssuschemeng.1c00781" target="_blank" >https://pubs.acs.org/doi/10.1021/acssuschemeng.1c00781</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acssuschemeng.1c00781" target="_blank" >10.1021/acssuschemeng.1c00781</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multifunctional 3D Hierarchical Bioactive Green Carbon-Based Nanocomposites

Popis výsledku v původním jazyce

Carbon-based nanocarriers such as multiwall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) have shown promising delivery capabilities due to their low immunogenicity, superior internalization, and suitable cell penetration efficiency. Herein, a molecular engineering strategy is advanced for the one-pot synthesized rGO/MWCNT/Fe3O4/ZnO to enhance the stability of the nanocarrier in the biological matrix; green synthesized ZnO was responsible for water uptake and reduced cytotoxicity, while Fe3O4 controlled the cellular internalization for gene delivery. Surface morphology of the ensuing nanocomposite was correlated with photocatalytic and gene delivery (CRISPR/Cas9) features. For the first time, a complete physical interaction between CRISPR/Cas9 and nanomaterial is evidenced via atomic force microscopy (AFM), demonstrating an increase in green fluorescence protein (EGFP) up to 11%. Furthermore, the enhanced photocatalytic activity is displayed in complete degradation of the methylene blue dye under 10 min with an efficiency of over 98%. The cytotoxicity of the nanocomposite is enhanced by ZnO on treatment with the PC12 and HEK-293 cell lines subsequent to 24, 48, and 72 h of exposure, with more than 88, 79, and 80% cell viabilities for PC12 and more than 88, 80, and 85% cell viabilities for HEK-293 in the maximum ratio of material to CRISPR (WR of nM/CC being 100). Furthermore, the nanocomposite showed an antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria (MZI values of 24 and 21 mm, respectively). The surface chemistry of the optimized system opens up new prospects to codeliver therapeutic agents for useful clinical applications.

Název v anglickém jazyce

Multifunctional 3D Hierarchical Bioactive Green Carbon-Based Nanocomposites

Popis výsledku anglicky

Carbon-based nanocarriers such as multiwall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) have shown promising delivery capabilities due to their low immunogenicity, superior internalization, and suitable cell penetration efficiency. Herein, a molecular engineering strategy is advanced for the one-pot synthesized rGO/MWCNT/Fe3O4/ZnO to enhance the stability of the nanocarrier in the biological matrix; green synthesized ZnO was responsible for water uptake and reduced cytotoxicity, while Fe3O4 controlled the cellular internalization for gene delivery. Surface morphology of the ensuing nanocomposite was correlated with photocatalytic and gene delivery (CRISPR/Cas9) features. For the first time, a complete physical interaction between CRISPR/Cas9 and nanomaterial is evidenced via atomic force microscopy (AFM), demonstrating an increase in green fluorescence protein (EGFP) up to 11%. Furthermore, the enhanced photocatalytic activity is displayed in complete degradation of the methylene blue dye under 10 min with an efficiency of over 98%. The cytotoxicity of the nanocomposite is enhanced by ZnO on treatment with the PC12 and HEK-293 cell lines subsequent to 24, 48, and 72 h of exposure, with more than 88, 79, and 80% cell viabilities for PC12 and more than 88, 80, and 85% cell viabilities for HEK-293 in the maximum ratio of material to CRISPR (WR of nM/CC being 100). Furthermore, the nanocomposite showed an antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria (MZI values of 24 and 21 mm, respectively). The surface chemistry of the optimized system opens up new prospects to codeliver therapeutic agents for useful clinical applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Sustainable Chemistry &amp; Engineering

ISSN

2168-0485

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

26

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

8706-8720

Kód UT WoS článku

000671060400005

EID výsledku v databázi Scopus

2-s2.0-85110274447