Natural Polymers Decorated MOF-MXene Nanocarriers for Co-delivery of Doxorubicin/pCRISPR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73612463" target="_blank" >RIV/61989592:15640/21:73612463 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acsabm.1c00332" target="_blank" >https://pubs.acs.org/doi/10.1021/acsabm.1c00332</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsabm.1c00332" target="_blank" >10.1021/acsabm.1c00332</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Natural Polymers Decorated MOF-MXene Nanocarriers for Co-delivery of Doxorubicin/pCRISPR

Popis výsledku v původním jazyce

A one-pot and facile method with assistance of high gravity was applied for the synthesis of inorganic two-dimensional MOF-5 embedded MXene nanostructures. The innovative inorganic MXene/MOF-5 nanostructure was applied in co-delivery of drug and gene, and to increase its bioavailability and interaction with the pCRISPR, the nanomaterial was coated with alginate and chitosan. The polymer-coated nanosystems were fully characterized, and the sustained DOX delivery and comprehensive cytotoxicity studies were conducted on the HEK-293, PC12, HepG2, and HeLa cell lines, demonstrating acceptable and excellent cell viability at both very low (0.1 mu g.mL(-1)) and high (10 mu g.mL(-1)) concentrations. The chitosan-coated nanocarriers showed superior relative cell viability compared to others, more than 60% on average of relative cell viability in all of the cell lines. Then, alginate-coated nanocarriers ranked at second place on the higher relative cell viability, more than 50% on average for all of the cell lines. Also, MTT results showed a complete dose-dependence, and by increasing the time of treatment from 24 to 72 h, the relative cell viability decreased by a meaningful slope; however, this decrease was optimized by coating the nanocarrier with chitosan and alginate. The nanosystems were also tagged with pCRISPR to analyze the potential application in the co-delivery of drug/gene. CLSM images of the HEK-293 and HeLa cell lines unveiled successful delivery of pCRISPR into the cells, and the enhanced green fluorescent protein (EGFP) reached up to ca. 2.6% for the HeLa cell line. Also, a considerable drug payload of 35.7% was achieved, which would be because of the interactions between the nanocarrier and the doxorubicin. In this unprecedented report pertaining to the synthesis of MXene assisted by a MOF and high-gravity technique, the methodology and the optimized ensuing MXene/MOF-5 nanosystems can be further developed for the co-delivery of drug/gene in animal models.

Název v anglickém jazyce

Natural Polymers Decorated MOF-MXene Nanocarriers for Co-delivery of Doxorubicin/pCRISPR

Popis výsledku anglicky

A one-pot and facile method with assistance of high gravity was applied for the synthesis of inorganic two-dimensional MOF-5 embedded MXene nanostructures. The innovative inorganic MXene/MOF-5 nanostructure was applied in co-delivery of drug and gene, and to increase its bioavailability and interaction with the pCRISPR, the nanomaterial was coated with alginate and chitosan. The polymer-coated nanosystems were fully characterized, and the sustained DOX delivery and comprehensive cytotoxicity studies were conducted on the HEK-293, PC12, HepG2, and HeLa cell lines, demonstrating acceptable and excellent cell viability at both very low (0.1 mu g.mL(-1)) and high (10 mu g.mL(-1)) concentrations. The chitosan-coated nanocarriers showed superior relative cell viability compared to others, more than 60% on average of relative cell viability in all of the cell lines. Then, alginate-coated nanocarriers ranked at second place on the higher relative cell viability, more than 50% on average for all of the cell lines. Also, MTT results showed a complete dose-dependence, and by increasing the time of treatment from 24 to 72 h, the relative cell viability decreased by a meaningful slope; however, this decrease was optimized by coating the nanocarrier with chitosan and alginate. The nanosystems were also tagged with pCRISPR to analyze the potential application in the co-delivery of drug/gene. CLSM images of the HEK-293 and HeLa cell lines unveiled successful delivery of pCRISPR into the cells, and the enhanced green fluorescent protein (EGFP) reached up to ca. 2.6% for the HeLa cell line. Also, a considerable drug payload of 35.7% was achieved, which would be because of the interactions between the nanocarrier and the doxorubicin. In this unprecedented report pertaining to the synthesis of MXene assisted by a MOF and high-gravity technique, the methodology and the optimized ensuing MXene/MOF-5 nanosystems can be further developed for the co-delivery of drug/gene in animal models.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Applied Bio Materials

ISSN

2576-6422

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

5106-5121

Kód UT WoS článku

000664594300043

EID výsledku v databázi Scopus

2-s2.0-85106504872