Precision Fishing for Enzyme-like Materials: Identifying and Engineering Mesoporous Catalytic Sites for Ferroptosis Induction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F25%3A73631477" target="_blank" >RIV/61989592:15640/25:73631477 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27640/25:10258849

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acs.nanolett.5c02476" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.nanolett.5c02476</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.nanolett.5c02476" target="_blank" >10.1021/acs.nanolett.5c02476</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Precision Fishing for Enzyme-like Materials: Identifying and Engineering Mesoporous Catalytic Sites for Ferroptosis Induction

Popis výsledku v původním jazyce

The discovery of nanomaterials with enzyme-like activities, termed nanozymes, holds the potential to revolutionize traditional inhibitor-based therapies. However, precise identification of nanozymes for specific substrates and uncovering their catalytic domains remain significant challenges. Here, we developed a &quot;fishing&quot; method that utilizes AFM probes coated with substrate baits, functioning as fishing rods to enable in situ visualization of enzymatic reactions on nanomaterial surfaces. Through this approach, we identified four nanozymes, including graphene oxides (GOs) exhibiting lipoxygenase (LOX)-like activity. Notably, mesopores were identified as key catalytic domains on GO surfaces, guiding the engineering of tailored porous GOs (tp-GOs) with enhanced LOX-like activity. The optimized tp-GOs catalyzed ferroptosis in cancer cells and suppressed hepatic tumor growth and metastasis in mice. Our findings open new avenues in drug discovery, shifting the paradigm from enzyme inhibition to promoting specific biochemical reactions using nanozymes.

Název v anglickém jazyce

Precision Fishing for Enzyme-like Materials: Identifying and Engineering Mesoporous Catalytic Sites for Ferroptosis Induction

Popis výsledku anglicky

The discovery of nanomaterials with enzyme-like activities, termed nanozymes, holds the potential to revolutionize traditional inhibitor-based therapies. However, precise identification of nanozymes for specific substrates and uncovering their catalytic domains remain significant challenges. Here, we developed a &quot;fishing&quot; method that utilizes AFM probes coated with substrate baits, functioning as fishing rods to enable in situ visualization of enzymatic reactions on nanomaterial surfaces. Through this approach, we identified four nanozymes, including graphene oxides (GOs) exhibiting lipoxygenase (LOX)-like activity. Notably, mesopores were identified as key catalytic domains on GO surfaces, guiding the engineering of tailored porous GOs (tp-GOs) with enhanced LOX-like activity. The optimized tp-GOs catalyzed ferroptosis in cancer cells and suppressed hepatic tumor growth and metastasis in mice. Our findings open new avenues in drug discovery, shifting the paradigm from enzyme inhibition to promoting specific biochemical reactions using nanozymes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/EH22_008%2F0004587" target="_blank" >EH22_008/0004587: Technologie za hranicí nanosvěta</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NANO LETTERS

ISSN

1530-6984

e-ISSN

1530-6992

Svazek periodika

25

Číslo periodika v rámci svazku

29

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

"11356–11364"

Kód UT WoS článku

001529518100001

EID výsledku v databázi Scopus

2-s2.0-105010716382