Comparison of two embryogenic cell lines of silver fir (Abies alba Mill.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43110%2F14%3A00224619" target="_blank" >RIV/62156489:43110/14:00224619 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of two embryogenic cell lines of silver fir (Abies alba Mill.)

Popis výsledku v původním jazyce

The embryogenic cell lines L27 and L122 were initiated from different immature zygotic embryos of silver fir (Abies alba Mill.). No polymorphism was found between these two cell lines by DNA analysis, but embryogenic cell lines differed during proliferation and maturation process. Embryogenic tissue L27 has more quickly turned brown and necrotized after 27 days of proliferation. These cell lines differed also in number of mature somatic embryos obtained by maturation process. Production of cotyledonarysomatic embryos and regenerants was significantly higher in L122 cell line. Protein content was higher in cell line L122. It was confirmed that inhibitory substances from pre-cultivated somatic embryos L27 spreading to agar cultivation medium by simple diffusion cause greater inhibition of tester mycelial growth during subsequent co-cultivation, compared to somatic embryos L122.

Název v anglickém jazyce

Comparison of two embryogenic cell lines of silver fir (Abies alba Mill.)

Popis výsledku anglicky

The embryogenic cell lines L27 and L122 were initiated from different immature zygotic embryos of silver fir (Abies alba Mill.). No polymorphism was found between these two cell lines by DNA analysis, but embryogenic cell lines differed during proliferation and maturation process. Embryogenic tissue L27 has more quickly turned brown and necrotized after 27 days of proliferation. These cell lines differed also in number of mature somatic embryos obtained by maturation process. Production of cotyledonarysomatic embryos and regenerants was significantly higher in L122 cell line. Protein content was higher in cell line L122. It was confirmed that inhibitory substances from pre-cultivated somatic embryos L27 spreading to agar cultivation medium by simple diffusion cause greater inhibition of tester mycelial growth during subsequent co-cultivation, compared to somatic embryos L122.

Druh

D - Stať ve sborníku

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Zborník referátov z vedeckej konferencie: "Dendrologické dni v Arboréte Mlyňany SAV 2014"

ISBN

978-80-971113-2-8

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

207-210

Název nakladatele

Arboretum Mlyňany SAV

Místo vydání

Vieska nad Žitavou

Místo konání akce

Vieska nad Žitavou: Arboretum Mlyňany SAV

Datum konání akce

1. 1. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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