Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F11%3A43908604" target="_blank" >RIV/62156489:43410/11:43908604 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cryobiol.2011.04.004" target="_blank" >http://dx.doi.org/10.1016/j.cryobiol.2011.04.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2011.04.004" target="_blank" >10.1016/j.cryobiol.2011.04.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity

Popis výsledku v původním jazyce

In coniferous species, including Greek fir (Abies cephalonica Loud), the involvement of somatic embryo plants in breeding and reforestation programs is dependent on the success of long-term cryostorage of embryogenic cultures during clonal field testing.In the present study on Greek fir, we assayed the recovery, morphological characteristics and genetic fidelity of embryogenic cell lines 6 and 8 during proliferation and maturation after long-term cryostorage. Our results indicate successful recovery ofboth cell lines after 6 years in cryostorage. In the maturation phase, both cell lines were capable of producing somatic embryos although some differences were detected among experiments. However, these changes were more dependent on the differences inthe components of the maturation media or in the experimental set-up than on the long-term cryostorage. During both proliferation and maturation phases, the morphological fidelity of the embryogenic cultures as well as of the somatic embr

Název v anglickém jazyce

Long-term cryopreservation of Greek fir embryogenic cell lines: Recovery, maturation and genetic fidelity

Popis výsledku anglicky

In coniferous species, including Greek fir (Abies cephalonica Loud), the involvement of somatic embryo plants in breeding and reforestation programs is dependent on the success of long-term cryostorage of embryogenic cultures during clonal field testing.In the present study on Greek fir, we assayed the recovery, morphological characteristics and genetic fidelity of embryogenic cell lines 6 and 8 during proliferation and maturation after long-term cryostorage. Our results indicate successful recovery ofboth cell lines after 6 years in cryostorage. In the maturation phase, both cell lines were capable of producing somatic embryos although some differences were detected among experiments. However, these changes were more dependent on the differences inthe components of the maturation media or in the experimental set-up than on the long-term cryostorage. During both proliferation and maturation phases, the morphological fidelity of the embryogenic cultures as well as of the somatic embr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GK - Lesnictví

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cryobiology

ISSN

0011-2240

e-ISSN

—

Svazek periodika

63

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

17-25

Kód UT WoS článku

000292020800004

EID výsledku v databázi Scopus

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