Effects of cadmium and lead stress on somatic embryogenesis of coniferous species. Part II: Changes of thiol substances

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43110%2F17%3A43911274" target="_blank" >RIV/62156489:43110/17:43911274 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/17:43911274 RIV/62156489:43410/17:43911274

Výsledek na webu

<a href="https://dx.doi.org/10.1007/s11738-017-2441-6" target="_blank" >https://dx.doi.org/10.1007/s11738-017-2441-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11738-017-2441-6" target="_blank" >10.1007/s11738-017-2441-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of cadmium and lead stress on somatic embryogenesis of coniferous species. Part II: Changes of thiol substances

Popis výsledku v původním jazyce

Conifers are often used as biomarkers of industrial pollution; however, little is known about the effects of heavy metals on them because only a few species have been tested. The aim of this work was to investigate the effects of cadmium (Cd2+) and lead (Pb2+) at three different concentrations (50, 250, and 500 µM) on the detoxification potential of Abies alba and Picea abies embryogenic cell masses throughout the 21-day proliferation period. Embryogenic cell masses of A. alba and P. abies responded to treatment with cadmium and lead by inducing phytochelatins and their biosynthetic intermediates. With increasing heavy metal concentrations, glutathione was used for the synthesis of phytochelatins enabling the tissues to bind to heavy metal ions and thereby avoiding the production of reactive oxygen species. Lead in A. alba and cadmium in both species caused similar increases of all antioxidative thiol compounds; thus, similar mechanisms involving a heavy metal-induced stress response can be assumed. In P. abies, the lowest lead concentration tested provoked the highest antioxidative response. Since a very low uptake of lead into the tissue was observed, the higher resistance of P. abies can be attributed to its ability to reduce lead uptake after longer exposure times. The results of cadmium treatment of both species and lead treatment of A. alba indicated the possibility of testing these coniferous species as potential phytoremediators. This is the first study to analyze the effects of heavy metals on the low-molecular-weight plant thiol content in A. alba embryogenic cell masses.

Název v anglickém jazyce

Effects of cadmium and lead stress on somatic embryogenesis of coniferous species. Part II: Changes of thiol substances

Popis výsledku anglicky

Conifers are often used as biomarkers of industrial pollution; however, little is known about the effects of heavy metals on them because only a few species have been tested. The aim of this work was to investigate the effects of cadmium (Cd2+) and lead (Pb2+) at three different concentrations (50, 250, and 500 µM) on the detoxification potential of Abies alba and Picea abies embryogenic cell masses throughout the 21-day proliferation period. Embryogenic cell masses of A. alba and P. abies responded to treatment with cadmium and lead by inducing phytochelatins and their biosynthetic intermediates. With increasing heavy metal concentrations, glutathione was used for the synthesis of phytochelatins enabling the tissues to bind to heavy metal ions and thereby avoiding the production of reactive oxygen species. Lead in A. alba and cadmium in both species caused similar increases of all antioxidative thiol compounds; thus, similar mechanisms involving a heavy metal-induced stress response can be assumed. In P. abies, the lowest lead concentration tested provoked the highest antioxidative response. Since a very low uptake of lead into the tissue was observed, the higher resistance of P. abies can be attributed to its ability to reduce lead uptake after longer exposure times. The results of cadmium treatment of both species and lead treatment of A. alba indicated the possibility of testing these coniferous species as potential phytoremediators. This is the first study to analyze the effects of heavy metals on the low-molecular-weight plant thiol content in A. alba embryogenic cell masses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

&quot;nestrankovano&quot;

Kód UT WoS článku

000403321400022

EID výsledku v databázi Scopus

2-s2.0-85019693378