Initiation, long-term cryopreservation, and recovery of Abies alba Mill. embryogenic cell lines
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F13%3A43908656" target="_blank" >RIV/62156489:43410/13:43908656 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s11627-013-9512-1" target="_blank" >http://dx.doi.org/10.1007/s11627-013-9512-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11627-013-9512-1" target="_blank" >10.1007/s11627-013-9512-1</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Initiation, long-term cryopreservation, and recovery of Abies alba Mill. embryogenic cell lines
Popis výsledku v původním jazyce
Somatic embryogenesis of Abies alba (Mill.) has significant potential to become an effective method for vegetative propagation of this species. To induce somatic embryogenesis in A. alba, the influence of the mother tree, sampling dates, and cold treatment storage of cones were examined. The initiation frequencies ranged from 1.7% to 16.6%. The sampling date and cone storage, but not the mother tree, had a significant effect on the initiation of embryogenic cultures. Storage of embryogenic cell lines was tested through cryopreservation for 6 yr. Four out of 12 cryostored embryogenic cell lines recovered, and the regeneration of cotyledonary embryos was obtained with two cell lines. The ability of embryogenic cell masses to produce somatic embryos and the mean number of cotyledonary embryos were higher when the maturation protocol was based on embryogenic suspensions dispersed on filter paper. The properly developed germinants were obtained only from maturation media where 32 mu M absci
Název v anglickém jazyce
Initiation, long-term cryopreservation, and recovery of Abies alba Mill. embryogenic cell lines
Popis výsledku anglicky
Somatic embryogenesis of Abies alba (Mill.) has significant potential to become an effective method for vegetative propagation of this species. To induce somatic embryogenesis in A. alba, the influence of the mother tree, sampling dates, and cold treatment storage of cones were examined. The initiation frequencies ranged from 1.7% to 16.6%. The sampling date and cone storage, but not the mother tree, had a significant effect on the initiation of embryogenic cultures. Storage of embryogenic cell lines was tested through cryopreservation for 6 yr. Four out of 12 cryostored embryogenic cell lines recovered, and the regeneration of cotyledonary embryos was obtained with two cell lines. The ability of embryogenic cell masses to produce somatic embryos and the mean number of cotyledonary embryos were higher when the maturation protocol was based on embryogenic suspensions dispersed on filter paper. The properly developed germinants were obtained only from maturation media where 32 mu M absci
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GK - Lesnictví
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
In Vitro Cellular & Developmental Biology-Plant
ISSN
1054-5476
e-ISSN
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Svazek periodika
49
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
560-571
Kód UT WoS článku
000326103900008
EID výsledku v databázi Scopus
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