Analýza avidinu a biotinu a jejich interakce na attomolové hladině pomocí voltametrických a chromatografických technik

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00000638" target="_blank" >RIV/62156489:43210/05:00000638 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/05:00021190

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

Popis výsledku v původním jazyce

The electroanalytical determination of avidin in solution, in a carbon paste, and in a transgenic maize extract was performed in acidic medium at a carbon paste electrode (CPE). The oxidative voltammetric signal resulting from the presence of tyrosine and tryptophan in avidin was observed using square-wave voltammetry. The process could be used to determine avidin concentrations up to 3 fM (100 amol in 3 mu l drop) in solution, 700 fM (174 fmol in 250 mu l solution) in an avidin-modified electrode, and174 nM in a maize seed extract. In the case of the avidin-modified CPE, several parameters were studied in order to optimize the measurements, such as electrode accumulation time, composition of the avidin-modified CPE, and the elution time of avidin. Inaddition, the avidin-modified electrode was used to detect biotin in solution (the detection limit was 7.6 pmol in a 6 mu l drop) and to detect biotin in a pharmaceutical drug after various solvent extraction procedures. Comparable studi

Název v anglickém jazyce

An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

Popis výsledku anglicky

The electroanalytical determination of avidin in solution, in a carbon paste, and in a transgenic maize extract was performed in acidic medium at a carbon paste electrode (CPE). The oxidative voltammetric signal resulting from the presence of tyrosine and tryptophan in avidin was observed using square-wave voltammetry. The process could be used to determine avidin concentrations up to 3 fM (100 amol in 3 mu l drop) in solution, 700 fM (174 fmol in 250 mu l solution) in an avidin-modified electrode, and174 nM in a maize seed extract. In the case of the avidin-modified CPE, several parameters were studied in order to optimize the measurements, such as electrode accumulation time, composition of the avidin-modified CPE, and the elution time of avidin. Inaddition, the avidin-modified electrode was used to detect biotin in solution (the detection limit was 7.6 pmol in a 6 mu l drop) and to detect biotin in a pharmaceutical drug after various solvent extraction procedures. Comparable studi

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

381

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

1167-1178

Kód UT WoS článku

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EID výsledku v databázi Scopus

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