Neutrofily tkáňového poolu mléčné žlázy skotu: ultrastrukturální vlastnosti během stárnutí in vitro.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00005394" target="_blank" >RIV/62156489:43210/05:00005394 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Tissue pool neutrophils of the bovine mammary gland: ultrastructural features during in vitro senescence.

Popis výsledku v původním jazyce

The aim of the study was to verify whether the in vitro senescence process of tissue pool neutrophils of the bovine mammary gland is accompanied by similar changes of ultrastructure as typically occurs in in vivo conditions. The experiments were carriedout in four clinically healthy, Holstein x Bohemian Red-Pied crossbred heifers aged 14 to 16 months. With the aid of TEM (transmission electron microscopy), SEM (scanning electron microscopy) and flow cytometry (FCM), neutrophil apoptosis in vivo was detected and during the senescence it was monitored in vitro. The neutrophil apoptosis comprised three ultrastructurally different stages: 1) karyopyknosis, 2) zeiosis, and 3) apoptotic bodies. These stages were obvious in the apoptotic neutrophils both invivo and in vitro. In addition to the common morphological signs, however, ultrastructural differences were also detected in apoptotic neutrophils in vitro. These in vitro ultrastructural differences mostly comprised hyper-vacuolation of

Název v anglickém jazyce

Tissue pool neutrophils of the bovine mammary gland: ultrastructural features during in vitro senescence.

Popis výsledku anglicky

The aim of the study was to verify whether the in vitro senescence process of tissue pool neutrophils of the bovine mammary gland is accompanied by similar changes of ultrastructure as typically occurs in in vivo conditions. The experiments were carriedout in four clinically healthy, Holstein x Bohemian Red-Pied crossbred heifers aged 14 to 16 months. With the aid of TEM (transmission electron microscopy), SEM (scanning electron microscopy) and flow cytometry (FCM), neutrophil apoptosis in vivo was detected and during the senescence it was monitored in vitro. The neutrophil apoptosis comprised three ultrastructurally different stages: 1) karyopyknosis, 2) zeiosis, and 3) apoptotic bodies. These stages were obvious in the apoptotic neutrophils both invivo and in vitro. In addition to the common morphological signs, however, ultrastructural differences were also detected in apoptotic neutrophils in vitro. These in vitro ultrastructural differences mostly comprised hyper-vacuolation of

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F03%2F1531" target="_blank" >GA524/03/1531: Role mononukleárních fagocytů během rezoluce akutního zánětu mléčné žlázy skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anatomia, histologia, embryologia

ISSN

0340-2096

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

159-166

Kód UT WoS článku

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EID výsledku v databázi Scopus

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