Vliv některých induktorů migrace leukocytů do mléčné žlázy skotu na apoptózu neutrofilů v následné in vitro kultivaci.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00006911" target="_blank" >RIV/62156489:43210/05:00006911 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/05:06G00201

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation

Popis výsledku v původním jazyce

The aim of the study was to elucidate the effect of induced leukocytes migration into bovine mammary gland on the manifestations of early and late apoptotic features of neutrophils cultivated in vitro. The neutrophil early apoptotic features were detected by the cytometric detection (FCM) phosphatidyl-serine translocation and DNA fragmentation. Late neutrophil apoptotic features were detected by ELISA quantitation histon-complexed DNA fragments. Leukocyte influx induction was accomplished by using fourinducers: i) sterile buffered saline solution (PBS); ii) 5% glucose solution (GLU); iii) synthetic muramyl dipeptide analogue (MDP); and iv) lipopolysaccharide (LPS), administered into the mammary gland lumen. After three-hour incubation, light microscopy examination revealed the highest statistically significant (P<0.05) expression of neutrophil apoptosis after induction by GLU. The lowest, though statistically insignificant apoptosis manifestation was detected by MDP and LPS. Determina

Název v anglickém jazyce

Effects of certain inducers of leukocytes migration into the bovine mammary gland on neutrophil apoptosis manifestation in a subsequent in vitro cultivation

Popis výsledku anglicky

The aim of the study was to elucidate the effect of induced leukocytes migration into bovine mammary gland on the manifestations of early and late apoptotic features of neutrophils cultivated in vitro. The neutrophil early apoptotic features were detected by the cytometric detection (FCM) phosphatidyl-serine translocation and DNA fragmentation. Late neutrophil apoptotic features were detected by ELISA quantitation histon-complexed DNA fragments. Leukocyte influx induction was accomplished by using fourinducers: i) sterile buffered saline solution (PBS); ii) 5% glucose solution (GLU); iii) synthetic muramyl dipeptide analogue (MDP); and iv) lipopolysaccharide (LPS), administered into the mammary gland lumen. After three-hour incubation, light microscopy examination revealed the highest statistically significant (P<0.05) expression of neutrophil apoptosis after induction by GLU. The lowest, though statistically insignificant apoptosis manifestation was detected by MDP and LPS. Determina

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F01%2F1290" target="_blank" >GA524/01/1290: Vliv antimikrobiálních látek na indukci apoptózy a nekrózy polymorfonukleárních leukocytů v rezoluci zánětu mléčné žlázy skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

305-312

Kód UT WoS článku

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EID výsledku v databázi Scopus

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