Spontánní a indukovaná cytolýza leukocytů mléčné žlázy skotu v případě kultivace in vitro - korelace s apoptózou neutrofilních granulocytů.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F06%3A00093531" target="_blank" >RIV/62156489:43210/06:00093531 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Spontaneous and induced cytolysis of leukocytes from bovine mammary gland in course of cultivation in vitro -- the correlation with neutrophil granulocytes apoptosis

Popis výsledku v původním jazyce

The process o fleukocyte cytolysis and the manifestations of apoptosis and secondary necrosis of neutrophil granulocytes (hereafter only "neutrophils") were studied on four virgin heifers after the induction of leukocyte influx into the mammary glandandafter their lavage in in vitro conditions. Phosphate buffered saline,muramyl dipeptide and a lipopolysaccharide were used for influx induction.Cytolys is and apoptosis were induced with heat stress, ultraviolet irradiation and spontaneous aging for 24 hours. The cytolysis was detected indirectly by determining the lactate dehydrogenase activity in the cultivation medium after the enzyme was released through cell lysis. The neutrophil apoptosis was detected using flowcytometry and two staining methods(i)simultaneous staining with Annexin V labelled FITC and propidium iodide and (ii) with SYTO 13. It was found that leukocytes of the mammary gland of virgin heifers undergo spontaneous aging during in vitro incubation. The fraction of lysed

Název v anglickém jazyce

Spontaneous and induced cytolysis of leukocytes from bovine mammary gland in course of cultivation in vitro -- the correlation with neutrophil granulocytes apoptosis

Popis výsledku anglicky

The process o fleukocyte cytolysis and the manifestations of apoptosis and secondary necrosis of neutrophil granulocytes (hereafter only "neutrophils") were studied on four virgin heifers after the induction of leukocyte influx into the mammary glandandafter their lavage in in vitro conditions. Phosphate buffered saline,muramyl dipeptide and a lipopolysaccharide were used for influx induction.Cytolys is and apoptosis were induced with heat stress, ultraviolet irradiation and spontaneous aging for 24 hours. The cytolysis was detected indirectly by determining the lactate dehydrogenase activity in the cultivation medium after the enzyme was released through cell lysis. The neutrophil apoptosis was detected using flowcytometry and two staining methods(i)simultaneous staining with Annexin V labelled FITC and propidium iodide and (ii) with SYTO 13. It was found that leukocytes of the mammary gland of virgin heifers undergo spontaneous aging during in vitro incubation. The fraction of lysed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

—

Projekt

<a href="/cs/project/GA524%2F03%2F1531" target="_blank" >GA524/03/1531: Role mononukleárních fagocytů během rezoluce akutního zánětu mléčné žlázy skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Medicine Czech

ISSN

0375-8427

e-ISSN

—

Svazek periodika

51

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

13

Strana od-do

265-277

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—