Spontánní a indukovaná cytoýza leukocytů bovinní mléčné žlázy během kultivace in vitro - korelace s apoptózou neutrofilních granulocytů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F06%3A%230000086" target="_blank" >RIV/00027162:_____/06:#0000086 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Spontaneous and induced cytolysis of leukocytes from bovine mammary gland in the course of cultivation in vitro - the correlation with neutrophil granulocytes apoptosis

Popis výsledku v původním jazyce

The shere of lysed leukocytes rose in the course of the in vitro incubation. The influx induction by phosphate buffered saline resulted in the lowest incidence of cytolysis, the induction with lipopolysaccharide, in the highest incidence. Heat stress, unlike ultraviolet irradiation, resulted in a significant increase in the fraction of lysed leukocytes. Ultraviolet radiation induced neutrophil apoptosis in a dominant way. Highly significant positive correlation between the percentage of apoptotic neutrophils and the percentage of lysed leucocytes was shown. The proportion of necrotic neutrophils positively correlated with share of lysed leukocytes only after influx induction with the lipopolysaccharide. After the influx induction with phosphate buffered saline statistically significant negative correlation was demonstrated. Then, when the more leukocytes succumb to cytolysis, thus the smaller share of necrotic neutrophils can be detected by flow cytometry.

Název v anglickém jazyce

Spontaneous and induced cytolysis of leukocytes from bovine mammary gland in the course of cultivation in vitro - the correlation with neutrophil granulocytes apoptosis

Popis výsledku anglicky

The shere of lysed leukocytes rose in the course of the in vitro incubation. The influx induction by phosphate buffered saline resulted in the lowest incidence of cytolysis, the induction with lipopolysaccharide, in the highest incidence. Heat stress, unlike ultraviolet irradiation, resulted in a significant increase in the fraction of lysed leukocytes. Ultraviolet radiation induced neutrophil apoptosis in a dominant way. Highly significant positive correlation between the percentage of apoptotic neutrophils and the percentage of lysed leucocytes was shown. The proportion of necrotic neutrophils positively correlated with share of lysed leukocytes only after influx induction with the lipopolysaccharide. After the influx induction with phosphate buffered saline statistically significant negative correlation was demonstrated. Then, when the more leukocytes succumb to cytolysis, thus the smaller share of necrotic neutrophils can be detected by flow cytometry.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA524%2F03%2F1531" target="_blank" >GA524/03/1531: Role mononukleárních fagocytů během rezoluce akutního zánětu mléčné žlázy skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

13

Strana od-do

5

Kód UT WoS článku

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EID výsledku v databázi Scopus

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