Využití polčítačového zpracovnán obrazu, rozpouštěcí voltametrie a hmotnostní spektroskopie při studiu vlivu olova na růst a životaschopnost raných soamtických embryí smrku ztepilého (Picea abies /L./ Karst.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00056375" target="_blank" >RIV/62156489:43210/05:00056375 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.)

Popis výsledku v původním jazyce

Image analysis (IA) was used to determine the areas and circumferences of clusters of early somatic embryos (ESEs) of the Norway spruce (Picea abies /L./Karst.). Results obtained from IA were compared with the fresh weights of the ESE clusters and theiresterase activities. The areas of the ESE clusters correlated well with both the increases in fresh weight (R2=0.99) of the ESEs and their esterase activities (R2=0.99). In addition, we studied the viability of the ESEs, which was determined by (a) double staining with fluorescein diacetate and propidium iodide (the resulting fluorescence was quantified by IA) and (b) determining esterase activity using a spectrofluorimetric detector. The results obtained with IA and esterase assay were comparable (thedeviation between the tangents of the bisectors was 6.4%). IA was also used to study the effect of Pb--EDTA

Název v anglickém jazyce

Application of computer imaging, stripping voltammetry and mass spectrometry for study of the effect of lead (Pb-EDTA) on growth and viability of early somatic embryos of Norway spruce (Picea abies /L./ Karst.)

Popis výsledku anglicky

Image analysis (IA) was used to determine the areas and circumferences of clusters of early somatic embryos (ESEs) of the Norway spruce (Picea abies /L./Karst.). Results obtained from IA were compared with the fresh weights of the ESE clusters and theiresterase activities. The areas of the ESE clusters correlated well with both the increases in fresh weight (R2=0.99) of the ESEs and their esterase activities (R2=0.99). In addition, we studied the viability of the ESEs, which was determined by (a) double staining with fluorescein diacetate and propidium iodide (the resulting fluorescence was quantified by IA) and (b) determining esterase activity using a spectrofluorimetric detector. The results obtained with IA and esterase assay were comparable (thedeviation between the tangents of the bisectors was 6.4%). IA was also used to study the effect of Pb--EDTA

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

383

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

576-586

Kód UT WoS článku

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EID výsledku v databázi Scopus

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