Ustavení polárního transportu auxinu v axilárních pupenech hrachu uvolněných z apikální dominance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00084749" target="_blank" >RIV/62156489:43210/05:00084749 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Establishing of polar auxin transport in pea axillary buds released from apical dominance

Popis výsledku v původním jazyce

Apical dominance is a dominance of the shoot apex over the axillary buds. It is well-known that auxin accumulated in the apex is transported towards the roots by polar transport. The mechanism, whereby polar auxin transport in the shoot regulates outgrowth of axillary buds, is unknown. One of the possibilities is that shoot auxin transport inhibits polarization of the cells in axillary buds, disabling the export of auxin from the inhibited buds. We studied the expression patterns of genes involved in auxin transport and bud dormancy (AUX 1, PIN 1 and PsDRM1) and localization of the putative PsPIN1 protein using anti-AtPIN1 antibodies in axillary buds during release from apical dominance. Semi-quantitative RT-PCR revealed a decrease of PsDRM1 expressionafter decapitation or BA application to the axillary buds of intact plants. Expression of PsPIN1 and PsAUX1 increases already 2 hours after decapitation with a maximum of about 150% at 6 hours and also the application of BA dramatically

Název v anglickém jazyce

Establishing of polar auxin transport in pea axillary buds released from apical dominance

Popis výsledku anglicky

Apical dominance is a dominance of the shoot apex over the axillary buds. It is well-known that auxin accumulated in the apex is transported towards the roots by polar transport. The mechanism, whereby polar auxin transport in the shoot regulates outgrowth of axillary buds, is unknown. One of the possibilities is that shoot auxin transport inhibits polarization of the cells in axillary buds, disabling the export of auxin from the inhibited buds. We studied the expression patterns of genes involved in auxin transport and bud dormancy (AUX 1, PIN 1 and PsDRM1) and localization of the putative PsPIN1 protein using anti-AtPIN1 antibodies in axillary buds during release from apical dominance. Semi-quantitative RT-PCR revealed a decrease of PsDRM1 expressionafter decapitation or BA application to the axillary buds of intact plants. Expression of PsPIN1 and PsAUX1 increases already 2 hours after decapitation with a maximum of about 150% at 6 hours and also the application of BA dramatically

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GP522%2F02%2FD137" target="_blank" >GP522/02/D137: Molekulární analýza polárního transportu auxinu při uvolňování z apikální dominance u hrachu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biologia Plantarum, 2nd International Symposium "Auxins and Cytokinins in Plant Development", Book of Abstracts

ISBN

0006-3134

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

"S22"-"S22"

Název nakladatele

Institute of Experimental Botany, Academy of Sciences of the Czech Republic

Místo vydání

Prague 2005

Místo konání akce

Praha

Datum konání akce

7. 7. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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