Molecular analysis of outgrowth of axillary buds of pea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A21100008" target="_blank" >RIV/62156489:_____/03:21100008 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular analysis of outgrowth of axillary buds of pea

Popis výsledku v původním jazyce

Apical dominance is a dominance of the shoot apex over the axillary buds. It is well-known that auxin accumulated in the apex is transported towards the roots by polar transport. The mechanism, whereby polar auxin transport in the shoot regulates outgrowth of axillary buds, is unknown. One of the possibilities is that shoot auxin transport inhibits connection of the buds onto shoot vascular elements and inhibits polarization of the cells. To study the role of genes involved in polar auxin transport in our model plant - pea, we tried to find homologous genes for auxin cell membrane transporters ([i]PIN1[/i] and [i]AUX1[/i]). Further we studied the influence of exogenous application of plant hormones (auxin and cytokinin) on the expression pattern of a gene already known to play a role in axillary bud dormancy - [i]PsDRM1[/i]. Northern blot analyses using DIG-labeled probes were used to follow the time course of gene expression of [i]PsDRM1[/i] in axillary pea buds after decapitation.

Název v anglickém jazyce

Molecular analysis of outgrowth of axillary buds of pea

Popis výsledku anglicky

Apical dominance is a dominance of the shoot apex over the axillary buds. It is well-known that auxin accumulated in the apex is transported towards the roots by polar transport. The mechanism, whereby polar auxin transport in the shoot regulates outgrowth of axillary buds, is unknown. One of the possibilities is that shoot auxin transport inhibits connection of the buds onto shoot vascular elements and inhibits polarization of the cells. To study the role of genes involved in polar auxin transport in our model plant - pea, we tried to find homologous genes for auxin cell membrane transporters ([i]PIN1[/i] and [i]AUX1[/i]). Further we studied the influence of exogenous application of plant hormones (auxin and cytokinin) on the expression pattern of a gene already known to play a role in axillary bud dormancy - [i]PsDRM1[/i]. Northern blot analyses using DIG-labeled probes were used to follow the time course of gene expression of [i]PsDRM1[/i] in axillary pea buds after decapitation.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GP522%2F02%2FD137" target="_blank" >GP522/02/D137: Molekulární analýza polárního transportu auxinu při uvolňování z apikální dominance u hrachu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

7th International congress of plant molecular biology

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

1

Strana od-do

130-130

Název nakladatele

ISPMB

Místo vydání

Barcelona, Spain

Místo konání akce

Barcelona, Spain

Datum konání akce

23. 6. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—