Vliv izolačních technik na životnost neutrofilů krve skotu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F06%3A00094818" target="_blank" >RIV/62156489:43210/06:00094818 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/06:#0000078

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of isolation techniques on viability of bovine blood neutrophils

Popis výsledku v původním jazyce

The effect of selected isolation methods on the viability of neutrophil granulocytes (neutrophils) from the blood of healthy Holstein x Bohemian Red Pied crossbred heifers was evaluated. Two methods of neutrophil isolation were used: a) neutrophil isolation on the basis of hypotonic erythrocyte lysis (in two variants: after the erythrocyte lysis proper, the cells were centrifuged at either 200 g or 1000 g) b) neutrophil isolation with FACS Lysing Solution as the lysing agent. The viability of the isolated neutrophils was evaluated on the basis of apoptosis and necrosis. The results obtained with flow cytometry (FCM) suggest that, from the isolation techniques used, the method based on FACS Lysing Solution impaired the neutrophil viability least. Afterthe application of this method, 5.36 +- 2.15 % of neutrophils were apoptotic and 0.51 +- 0.12 % were necrotic. In contrast, if the hypotonic erythrocyte lysis was used, the proportion of apoptotic neutrophils amounted to 42.14 +- 7.12 % a

Název v anglickém jazyce

Effect of isolation techniques on viability of bovine blood neutrophils

Popis výsledku anglicky

The effect of selected isolation methods on the viability of neutrophil granulocytes (neutrophils) from the blood of healthy Holstein x Bohemian Red Pied crossbred heifers was evaluated. Two methods of neutrophil isolation were used: a) neutrophil isolation on the basis of hypotonic erythrocyte lysis (in two variants: after the erythrocyte lysis proper, the cells were centrifuged at either 200 g or 1000 g) b) neutrophil isolation with FACS Lysing Solution as the lysing agent. The viability of the isolated neutrophils was evaluated on the basis of apoptosis and necrosis. The results obtained with flow cytometry (FCM) suggest that, from the isolation techniques used, the method based on FACS Lysing Solution impaired the neutrophil viability least. Afterthe application of this method, 5.36 +- 2.15 % of neutrophils were apoptotic and 0.51 +- 0.12 % were necrotic. In contrast, if the hypotonic erythrocyte lysis was used, the proportion of apoptotic neutrophils amounted to 42.14 +- 7.12 % a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EA - Morfologické obory a cytologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

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Svazek periodika

75

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

343-353

Kód UT WoS článku

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EID výsledku v databázi Scopus

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