The impact of pre-analytical treatment and sorting on human neutrophil function
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F14%3A43875327" target="_blank" >RIV/60162694:G44__/14:43875327 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.agriculturejournals.cz/web/vetmed.htm?volume=59&type=volume#No" target="_blank" >http://www.agriculturejournals.cz/web/vetmed.htm?volume=59&type=volume#No</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The impact of pre-analytical treatment and sorting on human neutrophil function
Popis výsledku v původním jazyce
This study was designed to test the hypothesis that pre-analytical treatment of relatively fragile cells is more important for their viability and function than shear forces and decompression shocks on a flow cytometric sorter. Human neutrophils were enriched using different techniques ( sedimentation, hypotonic or ammonium chloride-mediated erythrocyte lysis) and their function was evaluated by measuring their capability of mounting oxidative burst. In contrast to other isolation techniques, neutrophils enriched by spontaneous sedimentation were found to be intact both in terms of their function and relative numbers within the leukocyte population; such preparations were thus run on a FACSAria cell sorter using 70, 85 and 100 mu m nozzles and the corresponding internal pressure values. The viability and function of sorted neutrophils with or without subsequent cultivation were re-evaluated using phagocytosis assays. Independent of the nozzle size and internal sorter pressure, the capa
Název v anglickém jazyce
The impact of pre-analytical treatment and sorting on human neutrophil function
Popis výsledku anglicky
This study was designed to test the hypothesis that pre-analytical treatment of relatively fragile cells is more important for their viability and function than shear forces and decompression shocks on a flow cytometric sorter. Human neutrophils were enriched using different techniques ( sedimentation, hypotonic or ammonium chloride-mediated erythrocyte lysis) and their function was evaluated by measuring their capability of mounting oxidative burst. In contrast to other isolation techniques, neutrophils enriched by spontaneous sedimentation were found to be intact both in terms of their function and relative numbers within the leukocyte population; such preparations were thus run on a FACSAria cell sorter using 70, 85 and 100 mu m nozzles and the corresponding internal pressure values. The viability and function of sorted neutrophils with or without subsequent cultivation were re-evaluated using phagocytosis assays. Independent of the nozzle size and internal sorter pressure, the capa
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Veterinární medicína
ISSN
0375-8427
e-ISSN
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Svazek periodika
59
Číslo periodika v rámci svazku
11
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
10
Strana od-do
543-552
Kód UT WoS článku
000349028300004
EID výsledku v databázi Scopus
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