Isolation techniques of neutrophils and peripheral blood mononuclear cells for the comparative experiments in humans and pigs model organisms in flow cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A43908804" target="_blank" >RIV/62156489:43210/14:43908804 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2014/mnet_2014_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation techniques of neutrophils and peripheral blood mononuclear cells for the comparative experiments in humans and pigs model organisms in flow cytometry

Popis výsledku v původním jazyce

The pig is due to the morfology of their bodies often used as a model organism from the experimental organisms for comparison with man. The aim of this study ist o determine which isolation technique can be used for comparative imunological experiments focused on human and porcine neutrophils and monocytes. In comparative studies, it is necessary that both cells compared species were isolated by the same technique. Selected isolation techniques are the first step for subsequent detection of selected parameters in flow cytometry and for cultivation of peripheral blood mononuclear cells to profit monocyte-derivated macrophages. For isolation of neutrophils were chosen these techniques: isolation of double density gradient centrifugation Histopaque 11191a 1077, isolation of double density gradient centrifugation Histopaque 11191 a 1077 after dextran and only dextran sedimentation. Monocytes were isolated by density gradient centrifugation Histopaque 1077, density gradient centrifugation

Název v anglickém jazyce

Isolation techniques of neutrophils and peripheral blood mononuclear cells for the comparative experiments in humans and pigs model organisms in flow cytometry

Popis výsledku anglicky

The pig is due to the morfology of their bodies often used as a model organism from the experimental organisms for comparison with man. The aim of this study ist o determine which isolation technique can be used for comparative imunological experiments focused on human and porcine neutrophils and monocytes. In comparative studies, it is necessary that both cells compared species were isolated by the same technique. Selected isolation techniques are the first step for subsequent detection of selected parameters in flow cytometry and for cultivation of peripheral blood mononuclear cells to profit monocyte-derivated macrophages. For isolation of neutrophils were chosen these techniques: isolation of double density gradient centrifugation Histopaque 11191a 1077, isolation of double density gradient centrifugation Histopaque 11191 a 1077 after dextran and only dextran sedimentation. Monocytes were isolated by density gradient centrifugation Histopaque 1077, density gradient centrifugation

Druh

D - Stať ve sborníku

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2014: Proceedings of International Ph.D. Students Conference

ISBN

978-80-7509-174-1

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

516-521

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

19. 11. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000362518300099